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用于结构和动力学研究的重组蛋白质生产:使用M.结核病α-methylacyl-CoA种族酶 (MCR) 的案例研究

Otsile O Mojanaga1, K Ravi Acharya1, Matthew D Lloyd1

  • 1Department of Life Sciences, University of Bath, Claverton Down, Bath, United Kingdom.

Methods in enzymology
|October 20, 2023
PubMed
概括

本研究介绍了一种方法,用于从Mycobacterium tuberculosis中产生未标记的α-methylacyl-CoA种族酶 (MCR),使用重组E. coli. 开发的协议使MCR和其他重组蛋白质在没有亲和标签的情况下进行大规模净化.

关键词:
阳离子交换色谱学 阳离子交换色谱学阴离子交换色谱学 阴离子交换色谱学再组合蛋白的表达和净化.再组合的大肠杆菌.这是一个SDS-PAGE页面.尺寸排除色谱学 尺寸排除色谱学α-Methylacyl-CoA 赛马体是一种赛马体.

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