准备用于蛋白质标签的重组"Split AEP"

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Muge Ma1, Simon T M Tang1, Matthew T Dickerson1

  • 1School of Chemistry, Cardiff University, Park Place, Cardiff, United Kingdom.

Methods in enzymology
|October 20, 2023
PubMed
概括

一个新的,精简的协议显著简化了Oldenlandia affinis asparaginyl ligase (OaAEP1) 蛋白质标签工具的准备. 这种改进的方法将净化时间缩短到6小时以下,并增强酶活性和均性.

关键词:
亚斯巴拉基尼尔内化酶.亚斯巴拉基尼尔结合酶 (Asparaginyl ligase) 是一种火的副产品火.在OaAEPEP的OaAEP.酸绑定是一种

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