相关实验视频
Updated: Jun 9, 2026
12:45
Mechanical Stimulation of Chondrocyte-agarose Hydrogels
Published on: October 27, 2012
11.7K
时间酶治疗以加强多个软骨微组织的重塑,使其成为结构性组织组织
Ross Burdis1,2,3, Xavier Barceló Gallostra1,2,3, Daniel J Kelly1,2,3,4
1Trinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin, D02 PN40, Ireland.
Advanced healthcare materials
|October 20, 2023
概括
使用融合微组织的软骨工程比单个细胞提供了优势. 时间酶治疗增强了微组织融合和重塑,创造了具有改进结构的仿生软骨移植.
科学领域:
- 生物医学工程 生物医学工程
- 组织工程是组织工程.
- 再生医学是一种再生医学.
背景情况:
- 无脚手架组织工程旨在通过模仿发育过程来创建生物仿真移植.
- 虽然功能性软骨组织已经经过工程设计,但在实现本地性结构和整合方面仍然存在挑战.
- 目前的方法通常依赖于大量的单个细胞,这可能会限制矩阵发育和组织组织.
研究的目的:
- 与使用单个细胞相比,通过融合多个软骨微组织来证明工程软骨的好处.
- 为了解决聚合工程中不完整的微组织重塑的局限性.
- 研究时间酶治疗在加速结构改造和改善软骨移植发育方面的疗效.
主要方法:
- 工程软骨移植通过多个软骨微组织的融合.
- 实施使用冠状腺酶-ABC的时间酶治疗,以促进微组织融合和重塑.
- 分析细胞外矩阵组成,组织表型,原组织和机械性质.
主要成果:
- 从融合的微组织中设计的软骨表现出更丰富的细胞外基质,更像海林的表型,与单个细胞的移植相比,形状忠实度更高.
- 酶治疗加速了结构重塑,促进了微组织之间强大的融合,并增强了区域组织的原网络的发展.
- 酶治疗调节了矩阵组成和组织表型,对组织力学影响较小.
结论:
- 微组织自我组织是设计大规模软骨移植的有效策略,具有定义的几何形状和高矩阵积累.
- 时间酶治疗可以克服在软骨组织工程中使用生物构建块的关键局限性,从而产生更具生物模拟性的移植.
- 这种方法为产生用于再生医学应用的先进软骨移植提供了一个有前途的方法.
相关概念视频
01:40Bone Remodeling
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
01:27Fractures: Bone Repair
Treatment for a fracture is based on the type of break, the bone affected, and the patient's age.
Minor fractures with no bone displacement are treated by immobilizing the fractured bone using a cast or splint. However, in the case of fractures with displaced bones, the broken bones are repositioned before immobilization to ensure successful healing without deformation and loss of function. The realignment of fractured bone ends is performed through a process called reduction. If the procedure...
Minor fractures with no bone displacement are treated by immobilizing the fractured bone using a cast or splint. However, in the case of fractures with displaced bones, the broken bones are repositioned before immobilization to ensure successful healing without deformation and loss of function. The realignment of fractured bone ends is performed through a process called reduction. If the procedure...
01:29Bone Disorders
Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
01:31Bone Remodeling and Repair
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...