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基于干细胞或无细胞的基因疗法在慢性细胞再生中:来自突体液的介质干细胞外体

Onur Uysal^1,2,3, Haya Erybeh^1,2,4, Mediha Canbek⁴

¹Cellular Therapy and Stem Cell Production Application and Research Centre, ESTEM, Eskisehir Osmangazi University, Eskisehir, Turkiye.

Current molecular medicine

|October 20, 2023

概括

人类突流体衍生中介质干细胞 (hSFMSC) 外体为软骨损伤提供无细胞疗法. 该研究确定了hsa-miR-155-5p作为通过基因治疗增强软骨再生的关键miRNA.

科学领域:

生物医学工程生物医学工程
再生医学是一种再生医学.
细胞生物学细胞生物学

背景情况:

软骨损伤是常见的关节疾病之一.
干细胞疗法是一种有前途的治疗软骨再生.
细胞疗法面临着某些挑战,这促使人们研究替代方法.

研究的目的:

研究人类突液衍生中干细胞 (hSFMSC) 外体作为软骨损伤的无细胞治疗替代方案.
为了确定特定的微RNA (miRNA) 和参与干细胞治疗软骨再生的基因.
通过结合干细胞疗法和基因疗法来提高软骨修复的有效性.

主要方法:

使用流细胞计,免疫细胞化学和差异化分析对介质干细胞 (MSC) 的表征.
在体外吸收分析以功能性地表征孤立的外体.
用RT-qPCR比较从hSF-MSC分化出来的肌肉细胞与成熟的人类肌肉细胞,并分析基因表达特征.

主要成果:

从hSF-MSCs分化出来的冠状细胞与成熟的人类冠状细胞相比,显示hsa-miR-155-5p的表达显著更高.
TGF信号通路和软体生成标记基因支持了这些发现.
hSF-MSCs及其衍生的外体体在软骨修复方面表现出潜力.

结论:

hSF-MSC及其外体是治疗软骨损伤的可行选择.
在新型基因疗法中,hsa-miR-155-5p可以被用作向miRNA.
向hsa-miR-155-5p可以增加治疗软骨损伤的疗效.

关键词:

软骨缺陷是一种软骨缺陷.混凝土的生成混凝土的生成外基因组是外基因组的组成部分.胎儿人类冠状细胞细胞系.这是一个hsa-miR-155-5pp.突流体中介细胞干细胞

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