百祖-百绍草药对改善功能性便秘和肠道微生物群的疾病在老鼠
Xiaojun Li1, Xiaoting Wang1, Ziyan Wang1
1School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
根茎状虫 (Rhizoma atractylodis macrocephalae-Paeonia lactiflora Pall,简称BZBS) 通过改善肠道机动性和重新平衡肠道微生物群,有效治疗功能性便秘 (FC). 这项研究阐明了BZBS背后的机制.
科学领域:
- 传统中国医药 传统中国医药
- 胃肠病学 胃肠病学
- 微生物学 微生物学
背景情况:
- 树脂 (Rhizoma atractylodis macrocephalae-Paeonia lactiflora Pall,简称BZBS) 是一种传统的中国草药,用于治疗肠道疾病.
- 对于功能性便秘 (FC) 的BZBS的治疗机制在很大程度上仍未被阐明.
- 这项研究研究了BZBS对FC的老鼠模型的影响.
研究的目的:
- 阐明BZBS缓解功能性便秘 (FC) 的机制.
- 评估BZBS对FC大鼠结肠神经递质表达和肠道微生物群组成的影响.
主要方法:
- 建立了一个功能性便秘 (FC) 鼠标模型,并用BZBS进行治疗.
- 评估了分泌参数,包括便水分和肠道推进.
- 与酶相关的免疫吸收试验 (ELISA) 测量了神经递质水平 (SP,VIP,5-HT).
- 高通量16S核糖体RNA (16SrRNA) 基因测序分析了肠道微生物社区结构.
主要成果:
- 治疗BZBS显著改善了FC大鼠的关键分泌参数.
- 在结肠中,BZBS增加了物质P (SP),血管活性肠 (VIP) 和5-三胺 (5-HT) 的表达.
- BZBS改变了肠道微生物群的组成,降低了Bacteroidetes/Firmicutes的比率,并调节了特定属的丰富性 (例如,Blautia,Fusicatenibacter,Ruminococcus,Roseburia).
结论:
- 在大鼠模型中,BZBS在缓解功能性便秘 (FC) 方面表现出有效性.
- BZBS通过调节结肠神经递质水平和改善肠道失调来发挥其治疗作用.
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