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通过差分移动性光谱学改善类固醇的LC-MS/MS测量

Yubo Chai¹, Stefan K G Grebe^1,2,3, Anthony Maus¹

¹Department of Laboratory Medicine and Pathology, Division of Clinical Biochemistry and Immunology, Mayo Clinic, Rochester, MN 55905, USA.

Journal of mass spectrometry and advances in the clinical lab

|October 20, 2023

概括

差分移动光谱法 (DMS) 通过减少干扰和改善信号与噪声比来增强类固醇测量. 这种技术为具有挑战性的临床试验提供了更好的特异性和分析性能.

科学领域:

分析化学分析化学
临床诊断临床诊断临床诊断
质谱测量质量谱测量

背景情况:

类固醇测量对于诊断和监测各种健康状况和治疗非常重要.
类固醇之间的结构相似性带来了重大的分析挑战,即使对于像液态染色学-双重质谱法 (LC-MS/MS) 这样的先进技术.
差分移动谱法 (DMS) 提供了一个直角分离技术来补充现有方法.

研究的目的:

评估DMS在改善信号噪声比 (S/N) 和减少类固醇测量干扰方面的潜力.
使用LC-DMS-MS/MS.进行皮质醇和皮质素在尿液中的测量临床验证.

主要方法:

对六种不同的类固醇的DMS和没有DMS的染色体图进行比较分析.
使用液体染色学-差分移动性光谱学-并列质谱法 (LC-DMS-MS/MS) 方法在尿液中临床验证皮质醇和皮质.

主要成果:

DMS显著减少了干扰,并增加了S/N的1.6到13.8倍.
对于皮质醇和皮质,量化剂/合格剂碎片离子之间的一致性得到改善 (R2从~0.81增加到0.98).
验证研究以极佳的准确度 (%CVs <8%) 满足了验收标准.

结论:

通过减少干扰并增强S/N,DMS提高了类固醇测量的特异性.
DMS的好处不会影响分析性能的其他关键方面.
DMS显示了改善超越类固醇测量范围的广泛临床LC-MS/MS分析的希望.

关键词:

差分移动性光谱法差分移动性光谱法离子流动性的离子流动性这是LC-MS/MS.类固醇是一种类固醇.协奏式质谱测量质谱测量

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