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长非编码RNA Linc01224 通过与miR-485-5p和IGF2BP3的相互作用调节下甲状腺瘤的生长

Lai Wei^1,2, Yuanhang Wu³, Sisi Cai²

¹Department of Otolaryngology, The Eighth Affiliated Hospital of Sun Yat-sen University, 518033, Shenzhen, China.

Journal of Cancer

|October 20, 2023

概括

这项研究揭示了长非编码RNA linc01224通过调节miR-485-5p/IGF2BP3通路来促进下甲状腺皮细胞癌 (HSCC) 的进展. Linc01224是HSCC的潜在生物标志物和治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

长非编码RNAs (lncRNAs) 越来越多地被认为具有致癌作用.
在下甲状腺状细胞癌 (HSCC) 中 lncRNAs 的特定功能仍然不完全理解.
研究新的分子机制对于推进HSCC治疗至关重要.

研究的目的:

为了阐明link01224在HSCC中的作用.
在HSCC中调查linc01224,miR-485-5p和IGF2BP3的调节轴.
评估linc01224作为HSCC的潜在预后生物标志物和治疗点.

主要方法:

使用lncRNA测序和定量实时PCR (qRT-PCR) 来分析HSCC组织和细胞系中的linc01224表达.
在体外和体内进行了功能性实验,以评估 linc01224沉默的效果.
机制研究探讨了linc01224,miR-485-5p和IGF2BP3.3之间的相互作用.

主要成果:

与正常的邻近组织相比,Linc01224表达在HSCC组织中显著上调.
沉默linc01224抑制了瘤的扩散和迁移,并在HSCC中促进了亡.
发现Linc01224通过海绵化miR-485-5p积极调节IGF2BP3表达,促进HSCC的进展.
低IGF2BP3表达与HSCC患者的良好生存率相关.

结论:

Linc01224通过调节miR-485-5p/IGF2BP3轴作为HSCC中的瘤基因.
Linc01224显示出作为HSCC预后生物标志物的潜力.
向linc01224可能为HSCC提供一种新的治疗策略.

关键词:

低喉新生体的发生在 IGF2BP3BP3 中.长非编码RNA,长非编码RNA 链接0122424 在线阅读在 miR-485-5p 中使用.微RNAs 是一个微型RNA.瘤基因的基因基因.

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