相关实验视频

Updated: Jul 12, 2025

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

LncRNA MALAT1/microRNA-30b轴调节了巨细胞的两极分化和功能

Imran Ahmad¹, Raza Ali Naqvi¹, Araceli Valverde¹

¹Mucosal Immunology Lab, College of Dentistry, University of Illinois at Chicago, Chicago, IL, United States.

Frontiers in immunology

|October 20, 2023

概括

长非编码RNAMALAT1通过隔离microRNA-30b来促进促炎性巨细胞两极分化,影响免疫反应. 这项研究揭示了MALAT1在巨细胞功能和炎症中的作用.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
在RNA生物学,RNA生物学.

背景情况:

巨细胞 (Mφ) 是重要的免疫细胞,分化为M1 (前炎症) 或M2 (前溶解) 现型.
非编码RNAs,特别是长非编码RNAs (lncRNAs) 和microRNAs (miRNAs) 在巨细胞两极分化中的调节作用尚未完全理解.

研究的目的:

研究lncRNA MALAT1和microRNA miR-30b在调节巨细胞极化和免疫功能的功能相互作用.
阐明MALAT1影响巨细胞极化和先天免疫的机制.

主要方法:

巨细胞分化和两极分化试验.
定量实时PCR (qRT-PCR) 用于基因表达分析.
对MALAT1和miR-30b进行了淘汰和过度表达的研究.
双露西法酶记者测定证实了直接相互作用.
功能性检测包括抗原吸收,细胞和杀死细菌.
对人类牙周病和小鼠牙周炎模型的分析.

主要成果:

在巨细胞分化和LPS刺激过程中诱导MALAT1的表达.
抑制MALAT1促进M2巨细胞标记物,并损害M1标记物,这表明它有利于M1极化.
马拉特1倒置减少了巨细胞的菌和杀菌活动,并损害了细胞因子的分泌.
马拉特1直接与miR-30b相互作用,对抗其功能.
在人类和小鼠牙周炎组织中观察到高MALAT1和M1标志物与降低miR-30b,这表明体内有炎症作用.

结论:

该lncRNAMALAT1在促进M1巨细胞两极分化和抑制M2分化方面发挥着至关重要的作用.
马拉特1通过直接结合和封存对抗miR-30b,一个亲M2miRNA.
马拉特1有助于巨细胞功能中的炎症反应,并与牙周炎的发病有关.

关键词:

马拉特语马拉特语 lncRNA-miRNA相互作用的相互作用长非编码RNA是什么意思巨细胞的两极分化在 miR-30b 中使用.这是一个miRNA海绵.这是一个微型RNA.发酵细胞的形成发酵细胞的形成

更多相关视频

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Metabolic Characterization of Polarized M1 and M2 Bone Marrow-derived Macrophages Using Real-time Extracellular Flux Analysis

Published on: November 28, 2015

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays

Published on: April 18, 2025

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...