玉米母性哈普洛伊德诱导超出母系和ZmDMP的遗传基础
Henrique Uliana Trentin1,2, Matheus Dalsente Krause2, Rajkumar Uttamrao Zunjare2,3
1Bayer Crop Science, Coxilha, RS, Brazil.
Frontiers in plant science
|October 20, 2023
概括
研究人员发现了新基因,控制了玉米的平分体诱导能力. 这项工作促进了对影响单 haploid 诱导率 (HIR) 的遗传因素的理解,并有助于开发更有效的双 haploid (DH) 生产线.
科学领域:
- 植物遗传学和育种.
- 玉米的遗传学 玉米的遗传学
- 哈普洛伊德感应技术的技术.
背景情况:
- 玉米中双倍的平分体 (DH) 线对于加速育种计划至关重要.
- 孕产妇的平分体诱导剂用于*in vivo*DH生产线.
- 哈普洛伊德诱导率 (HIR) 是多基因的,目前只有少数几种基因被确定.
研究的目的:
- 识别控制玉米平分体诱导能力的新型基因和定量特征位点 (QTL).
- 为了深入了解基因架构的基因架构背后的HIR.
- 为提高DH生产线生产效率提供遗传资源.
主要方法:
- 全基因组关联研究 (GWAS) 使用~9007个单核酸多态 (SNP) 标记.
- 对159个母性单 haploid 诱导体的多样化小组的分析.
- 案例控制映射方法使用793个非诱导因子作为控制.
主要成果:
- 确定了MTL附近的一个主要基因,这对于破坏平分体诱导至关重要.
- 在染色体10上检测到一个显著的QTL,其中包含一个*kokopelli* ortholog.
- 在其他六个染色体上发现了额外的小效应的QTL,证实了多基因控制.
结论:
- 这项研究揭示了新的遗传因素,有助于玉米平分体诱导.
- 识别的QTL可以通过标记器辅助选择被整合到育种计划中.
- 通过基因进步改善HIR可以显著降低DH生产线的成本.
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