C型尿素通过抑制过度PINK1/Parkin介导的线粒细胞衰变来改善孕产妇年龄化的卵细胞质量
Hui Zhang1,2, Chan Li1,2, Qingyang Liu1,2
1College of Veterinary Medicine, Northwest A&F University, Yangling, China.
eLife
|October 20, 2023
概括
补充C型尿素 (CNP) 通过减少氧化损伤和恢复线粒体功能来改善老化的卵细胞质量. 这种方法可以提高助孕生殖成功率的妇女与晚年孕产妇.
科学领域:
- 生殖生物学 生殖生物学
- 分子内分泌学分子内分泌学
- 细胞衰老 细胞衰老
背景情况:
- 卵细胞质量随着年龄的增长而下降,这与活性氧物种 (ROS) 和氧化损伤的增加有关.
- 已知C型尿素 (CNP) 能改善不成熟卵子质量,但其在老化卵子中的作用尚不清楚.
研究的目的:
- 为了研究CNP在孕产妇年龄化的卵细胞中的作用.
- 为了确定CNP补充是否可以改善与年龄相关的卵细胞质量下降.
主要方法:
- 在老年小鼠中,血清中CNP水平和卵细胞质量的相关性分析.
- 用外源性CNP对老年小鼠的治疗.
- 评估卵细胞介质和细胞质成熟,受精能力,DNA损伤和亡.
- 卵细胞RNA测序 (RNA-seq) 用于分析分子机制.
- 对cAMP/PKA通路和PINK1/Parkin介导的线粒的研究.
主要成果:
- 血清中CNP水平与老年小鼠的卵细胞质量下降相关.
- CNP治疗改善了卵泡生长,排卵,介质/细胞质成熟和老化卵子中的受精.
- CNP改善了ROS诱导的DNA损伤和亡,并逆转了缺陷的表型.
- CNP恢复了线粒体的氧化酸化,减少了过度的线粒体,并调节了cAMP/PKA/PINK1/Parkin通路.
结论:
- 补充CNP是一种潜在的治疗策略,用于晚期的母亲与年龄相关的卵细胞恶化.
- CNP可以缓解氧化应激和过度线粒,改善卵细胞的质量和功能.
- 这一发现可能会提高老年妇女的辅助生殖结果.
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