相关实验视频
Updated: Jul 12, 2025

12:22
Biomimetic Materials to Characterize Bacteria-host Interactions
Published on: November 16, 2015
9.4K
来自Mobiluncus mulieris细菌的巨型粘合体中的域结构和交叉链接
Paul G Young1, Jacob M Paynter1, Julia K Wardega1
1School of Biological Sciences, The University of Auckland, Private Bag 92019, Auckland 1010, New Zealand.
Acta crystallographica. Section D, Structural biology
|October 20, 2023
概括
研究人员在Mobiluncus mulieris中发现了一种巨大的粘合物,这种细菌与细菌性阴道炎有关. 这种大型蛋白质具有独特的交叉链接,表明它在病原体引起感染的能力中起着关键作用.
科学领域:
- 微生物学 微生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 粘合素是细菌细胞表面蛋白质,对于殖民至关重要.
- 格拉姆阳性细菌通常利用粘合物中的分子内交叉链接.
- 摩拜 (Mobiluncus mulieris) 是一种与细菌性阴道炎相关的病原体.
研究的目的:
- 为了研究细菌粘附中的交叉链的流行和结构.
- 为了描述来自Mobiluncus mulieris. 的一个大型假定粘合物的特征.
- 确定这种粘合物的结构家族和潜在功能.
主要方法:
- 使用X射线晶体学和质谱学来分析特定的蛋白质域.
- 在剩余的蛋白质序列中使用AlphaFold结构预测.
- 进行了生物信息分析,以确定蛋白质家族和域.
主要成果:
- 鉴定了来自Mobiluncus mulieris的7651残留粘合物.
- 粘合素属于铁,异和含键 (TIE) 蛋白质家族.
- 它具有一个N端的硫粘附域和51个具有共价交叉链接的免疫球蛋白类域.
结论:
- 巨大的尺寸和复杂的交叉链接的M. mulieris粘附表明显著的能量投资由细菌.
- 这种独特的粘附可能在Mobiluncus mulieris的致病性和/或持续性中发挥着关键作用.
- 对TIE蛋白的进一步研究可能会揭示细菌粘附和毒性的新机制.
更多相关视频
相关概念视频
Adherens Junctions
4.8K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
4.8K
Fimbriae, Pili, and Axial Filaments
29
Fimbriae and pili are specialized bacterial surface structures that play pivotal roles in adhesion, genetic exchange, and motility. Composed primarily of pilin protein, these hairlike appendages are crucial for bacterial survival and pathogenicity in various environments.Fimbriae: Adhesion and PathogenicityFimbriae are fine, filamentous structures measuring 2–10 nanometers in diameter and are densely distributed on the bacterial cell surface. They facilitate bacterial adhesion to abiotic...
29
Structure of Cadherins
3.4K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
3.4K
Formation of Lipopolysaccharides
24
Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
24
Anchoring Junctions
3.8K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
3.8K
Immunoglobulin-like Cell Adhesion Molecules
3.3K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.3K

