在体外通过使用成年牛血清来增加介质细胞干细胞
Somia Shehzadi1, Maryam Javed2, Asmat Ullah3
1University Institute of Medical Laboratory Technology, The University of Lahore, 54000, Pakistan.
Current stem cell research & therapy
|October 20, 2023
概括
成人牛血清 (ABS) 有效地支持带介质干细胞 (UC-MSC) 的体外培养,为细胞扩张提供了胎儿牛血清 (FBS) 的可行和伦理替代方案.
科学领域:
- 干细胞生物学 干细胞生物学
- 生物技术是生物技术.
- 免疫学 免疫学 免疫学
背景情况:
- 带介质干细胞 (UC-MSCs) 由于免疫性较低,对免疫疗法充满希望.
- 目前的UC-MSC培养依赖于胎儿牛血清 (FBS),引发了伦理方面的担忧.
- 开发血清替代品对于可持续的UC-MSC扩张至关重要.
研究的目的:
- 评估成年牛血清 (ABS) 作为FBS在体外UC-MSC培养中具有成本效益和伦理性的替代品.
- 为了比较ABS与FBS在支持UC-MSC生长和扩散方面的疗效.
主要方法:
- 从牛带沃顿果中分离出UC-MSCs.
- 成人牛血清 (ABS) 被收集,热性无活化,并作为培养补充剂使用.
- 评估了UC-MSC形态,生长率,增殖指数和翻倍时间,并与FBS进行了比较.
主要成果:
- 在用ABS和FBS培养的UC-MSC之间观察到形态学和生长动力学的显著差异.
- ABS支持了强的UC-MSC扩张,与FBS相似.
结论:
- 成人牛血清 (ABS) 是一种有效的补充剂,用于UC-MSCs的体外扩张.
- 在干细胞培养中,ABS为FBS提供了一种实用和道德的替代方案.
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