链球菌的分子遗传特征 pyogenes 菌株从患有各种形式链球菌感染的患者中分离出来

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A G Minko¹, T A Danilova², G A Danilina²

¹Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya National Research Center of Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Moscow, Russia. alexi-m@yandex.ru.

Bulletin of experimental biology and medicine

|October 20, 2023

概括

这项研究分析了82种Streptococcus pyogenes菌株,发现了不同的emm类型和广泛的超抗原基因. 大多数菌株携带多个超抗原基因,显示与emm型相关的显著遗传变异.

科学领域:

微生物学微生物学
分子生物学分子生物学
传染性疾病传染性疾病

背景情况:

杆菌 pyogenes (A组杆菌) 导致各种感染.
了解S. pyogenes的遗传多样性,特别是其超抗原基因,对于疾病病原和流行病学至关重要.

研究的目的:

鉴定临床S. pyogenes分离物的emm类型和超抗原基因配置文件.
调查EMM类型与超抗原基因分布之间的关联.

主要方法:

在82个临床S. pyogenes菌株上进行了emm-typing.
多聚合物PCR和实时PCR被用于识别超抗原基因 (speC, speG, speH, speI, speJ, speK, speL, speM, smeZ, SSA, SpeA, SpeB).

主要成果:

确定了27种emm类型,其中emm-89,emm-75,emm-1是主要的.
所有菌株都含有超抗原基因,其中98%具有2-7个不同的基因.
观察到speG (93%) 和smeZ (98%) 的高患病率,以及其他超抗原基因分布的显著变异,与emm-type.type有一定的相关性.

结论:

临床分离的S. pyogenes表现出相当多样性的emm-类型和超抗原基因含量.
观察到的遗传变异性,特别是在超抗原基因组中,部分与特定的emm类型有关,影响S. pyogenes的致病性.

关键词:

在EMM-typing中使用.一组A型链球菌多次打印的PCR方法在实时PCR中进行实时PCR.它们是超级抗原.

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