相关实验视频

Updated: Jul 12, 2025

Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers

Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers

Published on: April 17, 2018

通过气相染色学-并联质谱学对2-基脂肪酸的全球分析揭示了皮动物中特定物种的丰富性

Lin Ding¹, Zhaozheng Chen¹, Yang Lu¹

¹Department of Biochemistry and Molecular Biology, Suzhou Medical College of Soochow University, Suzhou 215123, China.

Journal of agricultural and food chemistry

|October 20, 2023

概括

这项研究引入了一种新的GC-MS/MS方法,用于对疾病研究中至关重要的2-基脂肪酸 (2-OHFA) 进行分析. 在海黄瓜和海星中发现了高水平的2-OHFA,这表明了潜在的食应用.

科学领域:

分析化学分析化学
生物化学生物化学
海洋生物学海洋生物学

背景情况:

异常的2-基脂肪酸 (2-OH FAs) 水平与各种疾病有关.
在食品中定量2-OH FAs对于确定潜在的治疗补充的饮食来源至关重要.
由于结构复杂性和有限的标准,对2-OHFA进行全面的分析具有挑战性.

研究的目的:

开发一种创新的气体染色学-并联质谱法 (GC-MS/MS) 方法,用于全面的2-OH FA分析.
建立一种可靠的方法来识别和量化各种2-OH FA分子物种.
为了研究皮物种中的2-OH FA概况,以获得潜在的营养见解.

主要方法:

使用GC-MS/MS分析2-OH脂肪酸甲基 (FAMEs) 的三甲基 (TMS) 衍生物.
通过保留时间 (RT) 预测模型,使用签名碎片化模式进行峰值注释和结构确认.
开发了一种使用计划多重反应监测 (MRM) 的量化方法,并创建了50个2-OHFA的内部数据库.

主要成果:

在皮动物中成功分析了各种2-OHFA,包括海黄瓜 (Apostichopus japonicas) 和海星 (Asterias amurensis).
与海膽 (Mesocentrotus nudus, 531 ± 108 μg/g dw) 相比,海膽 (8.40 ± 0.28 mg/g dw) 和海星 (7.51 ± 0.18 mg/g dw) 的量化總二氧化FA含量顯著更高.
确定了2-OH C24:1作为海黄瓜中占主导地位的物种 (67.9%) 和2-OH C16:0作为海星中的主要物种.

结论:

开发的GC-MS/MS方法有效地表征了不同的2-OH FA分子物种,特别是海黄瓜和海星中丰富的分子物种.
这些发现突显了皮动物作为2-OHFA的丰富来源的潜力.
建议在未来开发使用这些海洋资源的养策略,以预防和治疗与2-OH FA缺乏相关的疾病.

关键词:

2-氧脂肪酸脂肪酸的使用方法在GC-MS中使用GC-MS.这是一个MRMM.皮动物皮动物回归模型是一种回归模型.

更多相关视频

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry

Qualitative Characterization of the Aqueous Fraction from Hydrothermal Liquefaction of Algae Using 2D Gas Chromatography with Time-of-flight Mass Spectrometry

Published on: March 6, 2016

Analysis of Fatty Acid Content and Composition in Microalgae

Analysis of Fatty Acid Content and Composition in Microalgae

Published on: October 1, 2013

相关概念视频

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

Mass Spectrometry: Long-Chain Alkane Fragmentation

Mass Spectrometry: Long-Chain Alkane Fragmentation

The molecular ions of linear alkanes prefer to fragment at the carbon-carbon bond away from the end of the chain since the cleavage of an inner bond creates a stable carbocation and a stable radical. Consequently, the mass signals of linear alkanes feature intense peaks in the middle of the mass-to-charge ratio plot with weaker peaks on either end. The fragmentation of each carbon-carbon bond with the release of a methyl group in each splitting leads to prominent peaks in the mass spectra...