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相关概念视频

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.1K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
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Proton (¹H) NMR: Chemical Shift01:07

Proton (¹H) NMR: Chemical Shift

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Organic molecules primarily contain carbon and hydrogen atoms. While all the hydrogen isotopes are NMR-active, protium or hydrogen-1 is the most abundant. It has a significant energy separation between its nuclear spin states due to its large gyromagnetic ratio. As per Boltzmann's distribution, an increase in the energy separation implies a greater excess population of nuclei available for excitation, resulting in a strong NMR absorption signal.
Absorption signals of all the protium nuclei...
1.7K
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

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In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
656
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.7K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
3.7K
¹H NMR of Labile Protons: Temporal Resolution01:10

¹H NMR of Labile Protons: Temporal Resolution

1.2K
Protons bonded to heteroatoms such as nitrogen and oxygen exhibit a range of chemical shift values. This is due to the varying degree of hydrogen bonding between the proton and the heteroatom in other molecules. The extent of hydrogen bonding affects the electron density around the proton, thereby giving different chemical shift values for the protons in the proton NMR spectrum.
The –OH proton in alcohols typically appears in the range of δ 2 to 5 ppm but can vary depending on the specific...
1.2K
¹H NMR of Labile Protons: Deuterium (²H) Substitution00:48

¹H NMR of Labile Protons: Deuterium (²H) Substitution

902
This lesson illustrates the role of deuterium substitution in simplifying the NMR spectrum of compounds comprising labile protons. One method employed is the use of deuterium. Amongst the three isotopes of hydrogen, deuterium (2H) has a nucleus composed of one proton and one neutron. When the D2O solvent is added to a pure dry ethanol solution, its labile proton is substituted with deuterium.
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相关实验视频

Updated: Jul 12, 2025

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
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使用无同位素标记的NMR进行蛋白质甲基化表征.

Zhongpei Fang1, Tao Huang2, Xin Chai1

  • 1Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement of Science and Technology, Chinese Academy of Sciences, Wuhan, 430071, China; University of Chinese Academy of Sciences, Beijing, 100049, China.

Talanta
|October 20, 2023
PubMed
概括

蛋白质甲基化在表观遗传学中至关重要,现在可以很容易地在没有同位素标记的情况下进行表征. 这种新方法揭示了癌症中具有变异性的基因组H3甲基化,有助于治疗的发展.

关键词:
节省了昂贵的成本,节省了成本.没有同位素的自由同位素.核磁共振光谱法 (NMR) 是一种光谱法.蛋白质甲基化蛋白质的甲基化.

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A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
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科学领域:

  • 生物化学 生化学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 在瘤学瘤学.

背景情况:

  • 蛋白质甲基化在表观遗传学中至关重要,也是癌症治疗的目标.
  • 目前用于测量甲基化的方法,如NMR,可能是昂贵和有限的.
  • 鉴定甲基化是理解甲基转移酶功能和开发抑制剂的关键.

研究的目的:

  • 开发一种简单的非同位素标记方法,用于表征蛋白质甲基化和脱甲基化.
  • 评估各种小鼠细胞和组织溶解体 (包括癌症细胞) 中的组织组织基因组H3甲基化模式.

主要方法:

  • 利用四个量子波器的1H-13C实验来选择性地检测甲基.
  • 应用了该方法来分析健康,癌症和瘤组织的溶解物中的基因组H3甲基化.
  • 没有要求对蛋白质或甲基捐赠者的同位素标记.

主要成果:

  • 在所有测试的小鼠溶解物中成功观察到基因素H3的单甲基和二甲基化.
  • 与癌症和健康细胞相比,在宫和乳腺瘤溶解物中发现了显著较低的H3甲基化率和水平.
  • 在不同细胞类型,组织和癌症阶段的H3甲基化模式中显示出可变性.

结论:

  • 开发的方法提供了一种简单的方法来表征蛋白质甲基化和脱甲基化.
  • 基因组H3甲基化模式在癌症中显著变化,为疾病机制提供了洞察力.
  • 这种技术有可能通过分析甲基化特征来开发有针对性的癌症干预措施.