ERβ-cAMP信号通路调节由雌醇诱导的绵羊卵细胞中介性停止
Bingxue Yang1, Yang An2, Yanyan Yang3
1College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 010018, PR China; Key Laboratory of Animal Embryo and Development Engineering of Autonomous Region Universities, Inner Mongolia Agricultural University, Hohhot, 010018, PR China.
Theriogenology
|October 20, 2023
概括
17β-雌二醇 (E2) 通过促进雌激素受体β (ERβ) 表达,增加循环腺单酸盐 (cAMP),维持绵羊卵细胞中介性停止. 这种对ERβ的cAMP正反澄清了E2的E2.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
背景情况:
- 17β-雌二醇 (E2) 和它的受体 (ERs) 对于卵细胞成熟至关重要,但精确的机制尚未完全理解.
- 了解E2及其受体在调节卵细胞中介性停止中的作用,对于改进体外成熟技术至关重要.
研究的目的:
- 研究ERα和ERβ在绵羊卵巢组织中的表达及其在卵细胞成熟中的作用.
- 阐明E2影响周期性腺单酸盐 (cAMP) 水平和绵羊卵细胞中中性进展的机制.
- 探索E2,cAMP和ERβ在维持中介性停止中的相互作用.
主要方法:
- 免疫组织化学检测羊卵巢组织中的ERα和ERβ蛋白表达.
- 卵细胞的体外成熟 (IVM) 具有不同的E2度和潜伏前时间.
- 艾丽莎测试用于体卵细胞复合体 (COC) 内cAMP水平,DAPI染色用于中介性进展,西部涂抹,以及实时PCR用于基因和蛋白质表达.
- 使用ERβ激动剂/抑制剂和cAMP调节剂 (Forskolin,IBMX,Rp-cAMP) 的药理学操纵.
主要成果:
- 两种ERα和ERβ蛋白都表达在绵羊的累积细胞和卵细胞中.
- E2治疗显著增加了COC内cAMP水平,维持了卵细胞中介性停止,并调高了ERβ表达.
- ERβ激活增强了E2诱导的cAMP增加,而ERβ抑制减弱了它;cAMP信号积极影响了ERβ表达.
结论:
- 低剂量E2 (0.5 ng/mL) 通过通过增强ERβ表达来增加COC内cAMP,维持绵羊卵细胞中介性停止.
- 在cAMP增强COC中的ERβ表达的地方存在一个积极的反循环.
- 这项研究提供了一个新的机制,解释了E2在绵羊毛囊发育和卵细胞成熟中的积极作用.
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