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相关概念视频

Cellulose and Pectic Polysaccharides01:15

Cellulose and Pectic Polysaccharides

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 Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth.  Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
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相关实验视频

Updated: Jul 12, 2025

Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
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黑卜根的性多糖:提取方法,结构特征

F V Vityazev1, V V Golovchenko1, O A Patova1

  • 1Institute of Physiology of Federal Research Centre "Komi Science Centre of the Urals Branch of the Russian Academy of Sciences", 50 Pervomaiskaya Str., 167982 Syktyvkar, Russia.

Food chemistry
|October 20, 2023
PubMed
概括

黑根含有大量的pectin,一个关键的多糖. 研究人员分离了pectin和粉,发现pectin是纸和皮的主要成分.

关键词:
核磁共振光谱法 (NMR) 是一种光谱法.质素 (Pectin) 是一种可怕的物质.拉法努斯·萨蒂斯 (Raphanus sativus var.) 是一种多种多样的植物. 尼格尔 (L.) 詹姆斯·克恩拉姆诺加拉克图罗南-I粉是一种粉.

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科学领域:

  • 植物生物化学 植物生物化学
  • 食品科学 食品科学 食品科学
  • 碳水化合物化学 碳水化合物化学

背景情况:

  • 黑卜 (Raphanus sativus L.) 的根是多糖的来源.
  • 了解这些多糖的组成对于潜在的应用很重要.

研究的目的:

  • 为了隔离和表征黑卜的粉和乳酸,从黑卜的根和皮.
  • 为了确定提取的pectins的产量和结构特征.

主要方法:

  • 使用各种溶剂 (水,氧酸盐,碳酸,氧化) 进行序列提取.
  • 用氨基葡萄糖酶和多糖酶进行酶降解.
  • 使用DEAE纤维素和通过NMR光谱学进行结构分析.

主要成果:

  • 氨氧酸盐溶液从纸和皮中产生了最高数量的多糖.
  • 标明是主要的多糖,在干燥物质上产量为9.5% (肉质) 和10.5% (皮质).
  • 核磁共振分析揭示了花蛋白结构中rhamnogalacturonan-I (RG-I) 和galacturonan (HG) 区域的存在,其中RG-I呈现出一个没有分支的结构.

结论:

  • 黑卜的根根是丰富的点丁来源,在皮皮中度更高.
  • 隔离的点含有不同的RG-I和HG区域,其中RG-I没有分支.
  • 这些发现有助于了解黑卜的成分和点的特征.