通过工程化Halomonas sp.的酸盐使用高效的聚基酸盐生产. JJY01 含有乙烯基-CoA 乙烯基转移酶
Jea-Kyung Park1, Jong-Min Jeon2, Yung-Hun Yang3
1Green & Sustainable Materials R&D Department, Korea Institute of Industrial Technology (KITECH), Cheonan-si 31056, Republic of Korea; School of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea.
International journal of biological macromolecules
|October 20, 2023
概括
工程化Halomonas细菌有效地消耗乙以产生聚基酸盐 (PHB),一种可生物降解的生物塑料. 引入乙-CoA乙转移酶 (atoAD) 在摇瓶和发酵器研究中显著提高了PHB产量和生产率.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物塑料生产 生物塑料生产
背景情况:
- 聚乙酸 (PHB) 是一种由微生物合成的可生物降解生物塑料.
- 乙酸,一种挥发性脂肪酸 (VFA),可以被Halomonas sp.利用. YLGW01用于生长和PHB生产.
研究的目的:
- 为了提高酸盐的利用率和PHB的产生,在Halomonas菌株中.
- 研究引入乙-CoA乙转移酶 (atoAD) 对酸盐消耗和PHB产量的影响.
主要方法:
- 基因工程的哈洛蒙纳斯 sp. 的基因工程. YLGW01通过在LacIq-Ptrc促销器下引入atoAD基因来创建Halomonas sp. JJY01.JJY01.JJY01.JJY01.JJY01.JJY01.JJJY01.JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ
- 在摇瓶和10L发酵器养的批量培养中,工程和野生型菌株之间的乙酸盐消耗和PHB生产的比较分析.
主要成果:
- 哈洛蒙纳斯 sp. 的一种. 在摇瓶研究中,JJY01显示了酸盐消耗率的增加 (0.27g/L/h vs 0.17g/L/h) 和PHB产量 (0.075g/g vs 0.016g/g).
- 在10L发酵器中进行的料批发发酵显示,Halomonas sp.的乙酸盐消耗率较高 (0.55g/L/h与0.35g/L/h),PHB产量较高 (0.091g/g与0.067g/g),PHB标位较高 (4.6g/L与2.9g/L). JJY01与野生类型相比.
- 改造的菌株显著提高了生物塑料生产能力.
结论:
- 引入atoAD显著提高了Halomonas菌株中的酸盐利用率.
- 工程制造的哈洛蒙纳斯 sp. JJY01提供了一个有前途的平台,用于高效和高产的生物降解生物塑料PHB从乙酸的生产.
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