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冷保护剂诱导的细胞内冰形成和结晶性在冷保存期间对细菌的影响

Pei Jiang¹, Qin Li², Baolin Liu³

¹Ministry of Education-Shanghai Key Laboratory of Children's Environmental Health, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.

|October 20, 2023

概括

合成防聚合物,如聚乙烯醇 (PVA),比甘提供更好的细菌冷保存. PVA和三糖的组合显著提高了S. thermophilus细菌的存活率.

科学领域:

微生物学微生物学
生物技术是生物技术.
材料科学材料科学材料科学

背景情况:

冷保存对于细菌的储存至关重要,但传统的冷保护剂,如甘油,可能是有毒的.
人工防聚合物正在被探索,作为减轻冷伤害和抑制冰形成的替代方案.

研究的目的:

为了比较各种合成防聚合物与糖醇对细菌冷保存的有效性.
调查不同冷保护剂组合对S. thermophilus的生存的影响.

主要方法:

评估了合成聚合物,包括聚乙烯酒精 (PVA),聚乙烯pyrrolidone,聚乙烯甘醇和德克斯与甘油相比.
使用糖醇,三糖和PVA-三糖组合评估了S. thermophilus的存活率.

主要成果:

与其他聚合物和甘油相比,聚乙醇 (PVA) 在降低冰的增长速度方面表现优越.
100毫克/毫升三和1毫克/毫升PVA+50毫克/毫升三的组合显著提高了S. thermophilus的存活率.

结论:

合成防聚合物,特别是PVA,显示出改善细菌冷保存的希望.
优化PVA和三糖的组合为S. thermophilus提供了一个优越的替代方案,而不是传统的糖冷保护.

关键词:

细菌的生存率细菌的生存率冰的增长抑制冰的增长抑制冰核形成的诱导过程

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