洞察Leguminosae植物的黄酸化物生物合成中缺少的化化步骤
Hao-Tian Wang1, Zi-Long Wang1, Kuan Chen1
1State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, 100191, China.
Nature communications
|October 20, 2023
概括
研究人员发现了GuApiGT,它是生产植物化物至关重要的酶. 这种酶有效地将粉添加到黄酸盐中,使得植物中的这些生物活性化合物的新生物合成成为可能.
科学领域:
- 生物化学 生物化学
- 植物科学 植物科学
- 代谢工程是代谢工程.
背景情况:
- 粉是生物活性植物化合物中发现的一种独特的分支链粉,称为粉酸.
- 化的生物合成,特别是化的阶段,尚不清楚.
- 黄酸糖化物是植物天然产品的主要类别.
研究的目的:
- 发现和描述参与化生物合成的新型化转移酶.
- 为了阐明糖供体选择性在化转移酶中的机制.
- 为了在异质系统中使类片的新生合成成为可能.
主要方法:
- 来自Glycyrrhiza uralensis的阿皮转移酶GuApiGT的基因发现和功能特征.
- 对化活性和基质特异性的酶分析.
- 对GuApiGT的X射线结晶学和结构分析.
- 蛋白质工程改变了糖供体选择性.
- 生物信息分析用于识别Leguminosae中的相关基因.
- 在Nicotiana benthamiana中异质表达和新生生物合成.
主要成果:
- 鉴定和表征了GuApiGT,它是一种有效的2′′-O-apiosyltransferase,用于类糖化物.
- 作为糖供体,GuApiGT对UDP-apiose表现出严格的选择性.
- 确定了GuApiGT的晶体结构,并确定了一个关键的糖结合基因 (RLGSDH).
- 具有改变糖选择性的工程GuApiGT突变.
- 在Leguminosae中发现了121个候选的apiosyltransferase基因,在功能上表征了四个.
- 在工程Nicotiana benthamiana中实现了flavonoid apiosides的新生合成.
结论:
- GuApiGT是一种高效的烯酸化转移酶,对化生物合成至关重要.
- 结构和生物化学分析揭示了GuApiGT糖供体选择性的基础机制.
- 这一发现使得能够在植物中进行化生产的工程.
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