新生儿的呼吸道微生物群被喘相关的致病细菌所定居
Jonathan Thorsen1, Xuan Ji Li2, Shuang Peng2
1COPSAC, Copenhagen Prospective Studies on Asthma in Childhood, Herlev and Gentofte Hospital, University of Copenhagen, Copenhagen, Denmark.
新生儿中特定细菌的早期呼吸道殖民与儿童喘风险有关. 一项研究发现,鼻子中的常见病原体,如肺炎链球菌 (Streptococcus pneumoniae),在7岁时预测了持续的喘息/喘.
科学领域:
- 微生物学 微生物学
- 儿科过敏和免疫学
- 基因组学就是基因组学.
背景情况:
- 儿童喘是一个越来越令人担忧的复杂的病因因素.
- 之前的研究将新生儿的呼吸道殖民与晚期喘发展联系起来,使用基于培养和测序的技术.
- 关于特定细菌种群,特别是无氧生物在喘病变发生过程中的作用存在差异.
研究的目的:
- 调查新生儿鼻微生物组与儿童喘风险之间的关联.
- 为了比较16S rRNA基因测序的鼻拭片与先前的培养数据和鼻测序的结果.
- 为了确定特定的细菌种群或社区结构,预测持续的喘息/喘.
主要方法:
- 16S rRNA基因测序 (V3-V4区域) 的鼻抽样从新生儿在COPSAC2000出生队列.
- 对细菌种群的相对丰富性的分析.
- 与现有的培养数据和来自COPSAC2010队列的下喉测序数据进行比较.
- 开发一个结合的病原体评分.
主要成果:
- 鼻中Streptococcus pneumoniae,Haemophilus influenzae和Moraxella catarrhalis的丰富性与鼻培养结果相关.
- 基于这些细菌的综合病原体评分与7岁时持续的喘息/喘有关.
- 涉及喘的厌氧菌 (Veillonella,Prevotella) 在鼻样本中检测得较少,但与病原体得分相关,表明复杂的社区动态.
结论:
- 特定的常见病原体对新生儿鼻结殖是持续性儿童喘的重要危险因素.
- 这些发现强调了早期呼吸道微生物组在喘发育中的重要性.
- 这些结果对制定有针对性的喘预防策略有影响.
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