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质内皮细胞和 podocytes 之间的交叉关系控制了它们对糖尿病病中代谢刺激的反应

Michael Albrecht^1,2, Carsten Sticht^3,4, Tabea Wagner^1,2

¹European Center for Angioscience (ECAS), Medical Faculty Mannheim of the University of Heidelberg, Ludolf-Krehl-Strasse 13-17, 68167, Mannheim, Germany.

Scientific reports

|October 20, 2023

概括

糖尿病病包括由于高葡萄糖和甲基醇的细胞变化. 细胞相互作用显著改变基因表达,影响疾病的进展.

科学领域:

腎臟病學 (nephrology) 是一種醫學專業.
分子生物学分子生物学
糖尿病学糖尿病学

背景情况:

糖尿病脏病 (DN) 涉及质内皮细胞 (GEC) 和 podocytes 的病理变化.
糖尿病中的代谢因素,如高血糖 (HG) 和甲基甘 (MGO),会影响这些细胞变化.
HG和MGO对GEC和 podocytes以及它们的细胞间通信的确切影响尚未完全理解.

研究的目的:

调查HG和MGO对GEC和细胞的独特和相互作用作用.
在糖尿病条件下识别这些细胞的关键转录性变化.
验证DN的相关动物模型中的发现.

主要方法:

建立了GECs和podocytes的共同培养系统,以模仿细胞间相互作用.
暴露在HG和MGO条件下的共同种植和单一种植.
通过基因表达分析评估和比较转录变化.
在BTBR ob/ob小鼠中验证了关键基因表达变化.

主要成果:

HG和MGO在基因表达上表现出明显的影响,在GEC和 podocytes之间存在差异.
HG上调即时早期反应基因 (例如EGR家族),炎症基因 (GECs) 和细胞循环基因 (podocytes).
无论是HG还是MGO都降低了细胞中的细胞外矩阵组织基因的下调;细胞细胞交叉声学显著调节了转录反应.

结论:

糖尿病代谢因子HG和MGO诱导GEC和细胞的特定分子变化.
GECs和podocytes之间的相互作用对于调解细胞对糖尿病刺激的反应至关重要.
这些发现提供了对DN病变的洞察力,并突出了细胞间通信的重要性.

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