相关实验视频

Updated: Jul 12, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

在质母细胞瘤中,TPPP3通过Snail1促进了表皮-介质母细胞过渡

Xu Xu¹, Yunan Hou¹, Niya Long¹

¹Department of Neurosurgery, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.

Scientific reports

|October 20, 2023

概括

结核素聚合促进蛋白3 (TPPP3) 在质母细胞瘤中升高,促进癌细胞迁移和增殖. 抑制TPPP3可能为质母细胞瘤患者提供新的治疗策略.

科学领域:

神经瘤学神经瘤学
癌症生物学癌症生物学

背景情况:

突聚合促进蛋白3 (TPPP3) 与各种癌症有关.
它在多形质母细胞瘤 (GBM) 中的具体作用和机制尚不清楚.

研究的目的:

研究TPPP3在质瘤中的作用和临床意义.
探索TPPP3作为GBM治疗点的潜力.

主要方法:

对质瘤组织中的TPPP3表达与正常脑组织 (NBT) 的比较分析.
在体外研究评估TPPP3上调和抑制对质母细胞瘤细胞行为 (迁移,入侵,增殖,亡) 的影响.
通过Snail1蛋白调节对TPPP3对表皮-半机细胞转换 (EMT) 的影响的分析.
在临床质母细胞瘤样本中对TPPP3和Snail1表达的相关性分析.

主要成果:

在质瘤中,TPPP3的表达明显高于NBT,并且随着瘤的等级而增加.
过度表达TPPP3增强了质母细胞细胞迁移,入侵,增殖,并减少了细胞亡.
抑制TPPP3可以逆转这些效应,从而诱导亡.
通过调节Snail1,TPPP3影响EMT,在质母细胞瘤中观察到TPPP3和Snail1蛋白水平之间存在正相关性.
较低的TPPPP3表达与改善的患者存活率相关.

结论:

在质母细胞瘤的进展中,TPPP3起着至关重要的作用.
TPPP3 是一种潜在的 glioma 级别和患者预后的生物标志物.
准TPPP3为新的质母细胞瘤疗法提供了一个有希望的途径.

更多相关视频

TGF-β-mediated Endothelial to Mesenchymal Transition EndMT and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing

TGF-β-mediated Endothelial to Mesenchymal Transition EndMT and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing

Published on: February 26, 2021

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

相关概念视频

Metastasis

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...