该BAF染色体重塑复杂许可平面干细胞访问外皮和中皮细胞命运
Mallory Wiggans1,2, Shu Jun Zhu1,2, Alyssa M Molinaro3
1The Hospital for Sick Children, Program in Developmental and Stem Cell Biology, Toronto, ON, M5G0A4, Canada.
BMC biology
|October 20, 2023
概括
BAF染色体重塑复合体对于平面成年干细胞 (ASC) 来分化为各种细胞类型至关重要,影响再生. 它的枯竭破坏了基因接入,影响了中皮和外皮血统的发展.
科学领域:
- 干细胞生物学 干细胞生物学
- 发育生物学是发展生物学.
- 染色体的调节 染色体调节
背景情况:
- 平面成年干细胞 (ASC),称为新芽细胞,具有自我更新和多能性.
- 染色体重塑复合体和转录因子调节基因表达和细胞命运.
- 以前的研究将BAF复杂成分RNAi与平面动物中ASC增加和再生失败联系起来.
研究的目的:
- 为了研究BAF染色体重塑复合体在平面神经母细胞中的作用.
- 了解BAF复杂子单元brg-1和smarcc2如何调节ASC中的基因表达和细胞命运.
主要方法:
- ATAC和RNA测序是在纯化的平面神经母细胞上进行的.
- 新芽细胞缺乏BAF复杂子单元brg-1和smarcc2通过RNAi.
- 基因可访问性和转录与BAF复杂功能相关分析.
主要成果:
- BAF复合物增强了平面神经细胞中基因的染色质可访问性和转录.
- 缺乏BAF复合体会影响对中皮和外皮谱系 (肌肉,神经,上皮) 发展至关重要的基因的获取.
- BAF复合物耗尽导致分化和再生中断,没有观察到对内皮系的影响.
结论:
- 在平面性ASC中,BAF复合体在调节细胞命运决定方面发挥着至关重要的作用.
- 这个复合体对于新芽细胞来说至关重要,它可以访问和转录特定血统分化所需的基因.
- 了解BAF复杂功能,可以了解干细胞调节和再生机制.
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