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在一个共同的参考空间中分析thalamocortical Tract-Tracing实验

Nestor Timonidis1, Mario Rubio-Teves2, Carmen Alonso-Martínez2

  • 1Neuroinformatics Department, Donders Centre for Neuroscience, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ, Nijmegen, The Netherlands. nestor.timonidis@donders.ru.nl.

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|October 21, 2023
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概括

这项研究引入了绘制小鼠大脑连接的新管道,详细介绍了来自腹腔后介质核 (VPM) 的甲状腺皮质投影. 该方法提高了地形精度,用于构建共享的虚拟大脑地图.

关键词:
一个共同的协调框架.在Connectomics上,我们提供了连接.皮层 皮层 皮层身体感官系统 身体感官系统索马托托皮 (Somatotopy) 是一种体外形的形式.空间登记是指空间登记.泰拉姆斯 泰拉姆斯 泰拉姆斯地形学地形学地形学地形学管道跟踪是指通道的跟踪.这是VPM VPM的意思.

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科学领域:

  • 神经科学是一个神经科学.
  • 大脑的连接性 大脑的连接性
  • 在Connectomics上,我们提供了连接.

背景情况:

  • 目前的中等层次连接图集缺乏在小鼠大脑中详细的甲状腺皮层投影信息.
  • 整合空间解析的thalamic神经元人口数据到一个共同的参考空间对于连接组分析至关重要.

研究的目的:

  • 开发和验证一个计算管道用于细分,注册,整合和分析多个通道追踪实验.
  • 调查七个神经元群体的轴突投射和内核组织,这些神经元群体位于 thalamus 的腹后中核 (VPM).

主要方法:

  • 一个新的管道转换了通道跟踪数据,以适应一个参考模板,以改善整合.
  • 在VPM中使用前级标记器标记七个神经元群体.
  • 数据分析包括 soma 位置映射和在皮质中的投影目标识别.

主要成果:

  • 在VPM中索马的位置与皮质中胡须,鼻子和嘴巴的表示相关.
  • VPM投射主要针对皮质的第4层.
  • 与现有地图集相比,开发的方法显示出更高的地形精度,结果与独立数据集保持一致.

结论:

  • 这项研究为达lamus的协作虚拟连接图谱奠定了基础.
  • 管道是用户友好的,可以通过Jupyter Notebook访问,并提供高级可视化工具.
  • 这种方法有助于研究各种乳头核的地形组织.