甲酸盐脱酶的工程,以改善二氧化碳转化到甲酸盐的转化潜力
Hong-Ling Shi1,2, Shu-Wei Yuan3, Xiao-Qi Xi2
1School of Bioengineering, Dalian University of Technology, 2 Linggong Road, Dalian, 116024, Liaoning, People's Republic of China.
World journal of microbiology & biotechnology
|October 21, 2023
概括
改造的格式脱酶 (FDH) 突变体显示了对减少二氧化碳的增强催化效率. 这些改进的酶为非光合作用二氧化碳转化和工业应用提供了更大的潜力.
科学领域:
- 生物化学 生化学
- 酶工程是什么? 酶工程是什么?
- 生物催化剂是一种生物催化剂.
背景情况:
- 形式脱酶 (FDH) 作为D-2-基酸脱酶起作用.
- FDH可以可逆性地减少CO2形成,作为非光合作用CO2还原酶.
研究的目的:
- 提高甲酸盐脱酶 (FDH) 的催化效率,以减少二氧化碳排放.
- 为了设计具有改善活动和稳定性的FDH突变体,用于二氧化碳转化.
主要方法:
- 用局部定向的突变发生法来制造两个FDH突变:V328I/F285W和V354G/F285W.
- 评估了酶活性,最佳温度和pH值,稳定性和运动参数 (kcat/Km).
- 使用计算机辅助方法阐明了改善特性的分子基础.
主要成果:
- 与母CbFDH^M2.2相比,突变体的还原活性大约是母CbFDH^M2.2的两倍.
- 对于相应的突变物来说,从二氧化碳中产生的甲酸盐的产量分别高出2.9倍和2.7倍.
- 突变体的最佳温度为55°C,活动增加在45°C和55°C之间.
- 突变者在9.0的最佳pH值和在4.0-11.5.5的pH范围内的优异稳定性.
- 突变体的kcat/Km值是原始酶的1.75倍.
结论:
- 改造的FDH突变体显示了显著提高的催化效率和稳定性,用于减少二氧化碳.
- 这些发现为进一步分子修改FDH提供了基础,以改善二氧化碳的捕获和转化.
- 改进的酶对非光合作用二氧化碳减排技术的应用具有前景.
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