控制不同根颜色的卜植物中安东素组成和含量的分子机制
Sun-Hyung Lim1, Da-Hye Kim1, Jong-Yeol Lee2
1Division of Horticultural Biotechnology, School of Biotechnology, Hankyong National University, Anseong, 17579, Republic of Korea; Research Institute of International Technology and Information, Hankyong National University, Anseong, 17579, Republic of Korea.
Plant physiology and biochemistry : PPB
|October 21, 2023
概括
卜根的颜色是由素成分决定的. 这项研究确定了调节这些色素的关键基因,包括调节这些色素的黄类3'-基酶 (RsF3'H) 和转录因子rsMYB1和rsTT8.
科学领域:
- 植物分子生物学 植物分子生物学
- 生物化学 生物化学
- 遗传学 是一个遗传学.
背景情况:
- 卜根的颜色因抗生素的形状而变化.
- 控制草根生物合成及其调节者的遗传机制尚未完全被理解.
研究的目的:
- 为了研究黄3亚酶 (RsF3H) 和转录因子RsMYB1和RsTT8在确定卜根颜色中的作用.
- 阐明不同颜色的卜根中安东素组成和含量的分子基础.
主要方法:
- 在四种卜品种中,RsF3H,RsMYB1和RsTT8的特征是具有独特根色 (白色,深红色,深紫色,深灰紫色) 的四种卜品种.
- 对安东素 (pelargonidin和cyanidin) 资料的分析.
- 促进子序列和促进子激活试验对RsMYB1和RsTT8.8进行比较.
主要成果:
- 深红色卜根由于低RsF3H表达积累了pelargonidin,而深紫色品种积累了高RsF3H表达的氨.
- RsMYB1和RsTT8转录存在于有色素的根,但不在白色根中.
- 在RSMYB1和RSTT8促进区的变异影响了基因表达,RSMYB1调节了自身表达和RSTT8表达.
结论:
- RsF3H对于卜根中的安东素组成至关重要.
- RsMYB1和RsTT8的表达水平是安东素含量的关键决定因素.
- 这项研究提供了对卜根中安东素多样性的调节的分子见解.
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