一个高效的双重功能光探针硫酸盐和硫化物及其成像应用在细胞中的应用
Bing-Yu Wei1, Cong-Yao Zhao2, Meng-Min Xiao1
1Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, PR China.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
|October 21, 2023
概括
一个新的双功能探头,SN-F,准确地监测细胞中的二氧化硫 (SO2) 衍生物和硫化 (H2S). 这种探测器对于了解相关疾病和开发新疗法至关重要.
科学领域:
- 生物化学 生物化学
- 化学生物学 化学生物学
- 细胞生物学 细胞生物学
背景情况:
- 二氧化硫 (SO2) 衍生物和硫化 (H2S) 是生理过程中的重要气体信号分子.
- 对细胞内的SO2衍生物和H2S度进行精确的监测对于疾病研究和治疗至关重要.
研究的目的:
- 设计和开发一种双功能探针,用于在生物系统中同时检测SO2衍生物和H2S.
- 评估探测器的性能,包括响应时间,选择性和在细胞环境中的适用性.
主要方法:
- 一个新的双功能探头,SN-F,是利用弗斯特共振能量转移 (FRET) 机制设计的.
- 探测器的性能被评估为SO2和H2S检测,包括响应动力学,选择性和线性.
- 进行了细胞成像实验,以评估探测器的准能力和对内源和外源H2S的监测能力.
主要成果:
- 在SN-F探测器上,SO2检测的快速响应时间为2分钟.
- 在非有机溶剂系统 (pH 7.4) 中,SN-F表现出优异的抗干扰能力和选择性,适合细胞SO2测定.
- 探测器显示了H2S检测的广泛线性范围,并成功地准了细胞成像的内质网膜 (ER),监测了内源和外源的H2S.
结论:
- 双功能SN-F探针是用于在细胞环境中准确量化SO2衍生物和H2S的宝贵工具.
- 在活细胞中,SN-F能够准ER并监测H2S的能力为研究这些气递质在健康和疾病中的作用开辟了新的途径.
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