相关实验视频

Updated: Jul 12, 2025

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

A Microphysiological System to Study Leukocyte-Endothelial Cell Interaction during Inflammation

Published on: December 9, 2021

Src 激酶部分调解了细胞因子诱导的内皮功能障碍

Amanda K Mauro¹, Luca Clemente¹, Nauman Khurshid²

¹Perinatal Research Laboratories, Department of Obstetrics & Gynecology, University of Wisconsin - Madison, School Medicine and Public Health, Madison, WI 53715, USA.

Pregnancy hypertension

|October 21, 2023

概括

Src 激酶通过影响信号传递和单层完整性,在孕前症中部分调解内皮功能障碍. 抑制Src激酶可以防止侮辱,这表明基于内皮细胞的治疗方法的治疗标.

科学领域:

血管生物学血管生物学
细胞信号传递细胞信号传递
孕前的病理生理学和病理学.

背景情况:

内皮功能障碍是孕前症 (PE) 的核心原因,有助于器官损伤.
增长因子和细胞因子在PE中失调内皮功能,影响 (Ca2+) 信号和屏障完整性.
生产是唯一的PE治疗方法,需要基于内皮细胞的新型治疗策略.

研究的目的:

调查Src酶在调解生长因子和细胞因子诱导的内皮功能障碍中的作用.
确定Src酶抑制是否可以在PE的细胞模型中逆转内皮功能障碍.

主要方法:

人类静脉内皮细胞 (HUVEC) 暴露于生长因子或细胞因子.
使用 (Ca2+) 图像和电池阻抗传感 (ECIS) 来评估内皮功能.
酶抑制剂 (Src 抑制剂 PP2,MEK/ERK 抑制剂 U0126) 用于阐明信号通路.

主要成果:

Src 激酶抑制保护HUVECs免受由VEGF165,bFGF,PlgF,TNFα和IL-1β引起的Ca2+信号干扰.
此外,Src抑制还保留了内皮单层对TNFα诱导的抗性.
抑制MEK/ERK并没有防止生长因子介导的内皮功能障碍.

结论:

细胞因子和生长因子刺激的 Src 激酶在 HUVEC 中部分促进内皮功能障碍.
向Src酶代表了与孕前相关的内皮功能障碍的潜在治疗策略.

关键词:

细胞因子细胞因子内皮内皮细胞内皮细胞内皮细胞内皮增长因子增长因子在HUVECEC中使用.孕前症孕前症这就是Src 激酶.

更多相关视频

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

Published on: April 21, 2023

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

相关概念视频

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

Receptor Tyrosine Kinases

Receptor Tyrosine Kinases

Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...

Amplifying Signals via Enzymatic Cascade

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...