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介质干细胞"条件介质对结直肠癌细胞系的亡效应

Roya Khamooshi¹, Ali Salimi², Raheleh Halabian³

¹Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University Tehran, Iran.

|October 21, 2023

概括

从脂聚糖原先条件的介质中制造的介质介质从脂聚糖原先条件的介质中制造的介质介质从脂聚糖原先条件的介质中制造的介质介质从脂聚糖原先条件的介质中制造的介质介质从脂聚糖原先条件的介质中制造的介质介质从脂聚糖原先条件的介质中制造的介质介质从脂聚糖原先条件的介质中制造的介质介质从脂聚糖原先条件的介质中制造的介质介质从脂聚糖原先条件的介质中制造的介质介质从脂聚糖原先条件的介质中制造的介质介质从脂聚糖原先条件的介质中制造的介质介质从脂聚糖原先条件的介质中制造的介质介质从脂聚糖原先条件的介质中制造的介质这表明一种潜在的新策略来抑制癌症的进展.

科学领域:

细胞生物学细胞生物学
在瘤学瘤学.
干细胞研究干细胞研究

背景情况:

介酶干细胞 (MSCs) 具有强大的免疫调节和亡特性.
MSCs可以迁移到炎症和瘤微环境中.
它们的条件介质 (CM) 正在探索癌症治疗中的治疗潜力.

研究的目的:

为了研究MSC条件介质 (CM) 对结直肠癌细胞系的亡效应.
评估MSC产生的脂聚糖诱导CM (LPS-CM) 对结直肠癌细胞 (HT29和SW48) 的疗效.

主要方法:

在MSC中使用脂聚糖 (LPS) 进行预条件,以产生LPS-CM.
结肠直肠癌细胞系 (HT29,SW48) 用LPS-CM进行治疗.
通过Trypan蓝色染色和MTT试验评估的细胞存活率和细胞增殖率.
通过流细胞计和caspase-3活性评估的亡.
使用实时PCR分析的bcl2,bax和p53的mRNA水平.

主要成果:

在SW48和HT29细胞中,LPS-CM显著增加了细胞亡 (p < 0.001).
在治疗的癌细胞中,caspase-3活性明显升高 (p < 0.001).
观察到bcl2mRNA的下调和bax和p53mRNA的显著上调 (p < 0.001).

结论:

来自LPS预先条件的MSCs的条件介质对结直肠癌细胞表现出显著的亡效应.
LPS-CM改变了关键的亡和抗亡基因 (bcl2, bax, p53) 的表达.
这表明LPS-CM是抑制结直肠癌的有前途的治疗策略.

关键词:

细胞灭亡 (apoptosis) 是一种死亡的过程.结肠直肠癌是一种癌症.有条件的介质介质.脂聚糖类糖化物脂聚糖类糖化物介质细胞干细胞介质细胞干细胞

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