TGFB1诱导胎儿重编程,并增强肠道再生
Lei Chen1, Xia Qiu2, Abigail Dupre2
1School of Life Science and Technology, Key Laboratory of Developmental Genes and Human Disease, Southeast University, Nanjing 210096, China.
Cell stem cell
|October 21, 2023
概括
转化生长因子β1 (TGFB1) 信号,由巨细胞介导,对于损伤后的肠道再生至关重要. TGFB1诱导胎儿般的再生状态,促进修复,并为细胞疗法提供潜力.
科学领域:
- 胃肠病学 胃肠病学
- 再生医学是一种再生医学.
- 分子生物学分子生物学
背景情况:
- 肠表皮在受伤后具有显著的自我修复能力.
- 了解驱动肠道再生的分子机制对于治疗的发展至关重要.
研究的目的:
- 为了确定关键的信号通路参与损伤后肠道再生.
- 阐明转化生长因子β (TGFB) 在这个过程中的作用.
主要方法:
- 高通量测序,小鼠遗传学和有机体模型 (老鼠和人类).
- 辐射诱导的肠损伤模型.
- 巨细胞枯竭和TGFB信号传递的遗传破坏.
主要成果:
- 辐射后2天观察到由巨细胞介导的TGFB1表达的激增.
- 在巨细胞枯竭或TGFB信号干扰后出现再生障碍.
- TGFB1治疗诱导了有机体中的胎儿样/再生转录特征,并增强了介质细胞的反应.
- 支持再生的YAP/TEAD和SOX9因子被TGFB1在表皮中激活.
结论:
- 由巨细胞协调的TGFB1信号传递对于肠道上皮质再生至关重要.
- TGFB1驱动着类似胎儿的再生状态,并激活关键的亲再生因素.
- TGFB1对增强肠道修复的细胞疗法充满希望.
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