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Updated: Jul 12, 2025

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

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Published on: October 21, 2022

一种新型的循环RNA,circSQSTM1,保护动脉样硬化中的内皮功能

Ziqi Chen¹, Ruoyu Wang², Yinghong Zhu¹

¹Department of Cardiology, Translational Research Center for Regenerative Medicine and 3D Printing Technologies, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510150, China.

Free radical biology & medicine

|October 21, 2023

概括

一种新型的循环RNA,circSQSTM1,通过上调Sirt1.1,保护内皮细胞免受动脉样硬化. 这一发现为治疗动脉样硬化提供了一个新的治疗点.

科学领域:

心血管生物学心血管生物学
分子生物学分子生物学
在RNA生物学,RNA生物学.

背景情况:

动脉样硬化 (AS) 是一种主要的心血管疾病,其特征是内皮细胞 (EC) 功能障碍.
内皮细胞在动脉样硬化的发展和进展中起着至关重要的作用.
需要针对EC的新型治疗策略来对抗AS.

研究的目的:

研究新型循环RNA,circSQSTM1在动脉样硬化期间内皮细胞中的作用.
阐明circSQSTM1影响EC功能和AS进展的分子机制.
为了确定circSQSTM1在动脉样硬化中的治疗潜力.

主要方法:

在阿托瓦斯塔丁刺激的EC中选circRNAs.
评估circSQSTM1对EC炎症,氧化应激和自的影响.
分子机制研究包括化酶记者测定,RNA免疫沉和FISH.
通过动脉样硬化的小鼠模型进行体内验证.

主要成果:

阿托瓦斯塔丁 (ATV) 通过抑制m6A修饰的降解,诱导circSQSTM1在ECs中的积累.
circSQSTM1通过细胞质 (miR-23b-3p海绵) 和核 (FOXO1 mRNA出口) 机制,通过EC中Sirt1表达的升级调节.
circSQSTM1减少了EC炎症,氧化应激,并促进了自.
在体内,circSQSTM1的过度表达会减轻动脉样硬化进展.

结论:

circSQSTM1是一种新型的非编码RNA,在内皮细胞中起着保护性作用,防止动脉样硬化.
circSQSTM1通过上调Sirt1来发挥其保护作用,从而减轻炎症,氧化应激,并促进自.
circSQSTM1代表了动脉样硬化的潜在治疗标.

关键词:

动脉样硬化是一种动脉样硬化.这是循环RNARNA.内皮细胞是内皮细胞.在Sirt1上 circSQSTM1 的一个字.

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