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长读测序通过针对标准和长工作流来揭示植物转录组的全部复杂性
Othman Al-Dossary1,2, Agnelo Furtado1, Ardashir KharabianMasouleh1
1Queensland Alliance for Agriculture and Food Innovation, University of Queensland, Brisbane, 4072, Australia.
Plant methods
|October 21, 2023
概括
在植物转录组分析中,包括标准和长转录库至关重要. 这种方法提高了基因组覆盖范围,并揭示了广泛的替代拼接,改善了转录的发现.
科学领域:
- 植物基因组学 植物基因组学
- 对转录组进行分析.
- 这是下一代测序.
背景情况:
- 长读测序提供了全长的植物转录分析,绕过了组装挑战.
- 通常使用大小转录库,但它们的比较价值尚不清楚.
研究的目的:
- 为了评估使用不同的转录库大小用于植物转录基因组测序的影响.
- 评估基因组覆盖和替代拼接检测的全面性.
主要方法:
- 使用两个PacBio库准备生成Jojoba (Simmondsia chinensis) 叶子转录组:标准工作流 (SW) 和长工作流 (LW).
- 从两种类型的库中分析和合并独特和共享的序列.
主要成果:
- 将SW和LW图书馆结合起来,产生了74.6%的独特序列,其中37.8%的更长的成绩单仅在LW数据集中.
- 实现了高的转录组完整性 (98.7%的预测基因) 和基因覆盖率 (96.9%的BUSCO)
- 确定了167,866种高质量的异构体,其中71.7%是蛋白质编码的,并揭示了替代拼接和内部保留作为关键的多样性驱动因素.
结论:
- 针对更长的转录对于在转录组研究中全面覆盖植物基因组至关重要.
- 这种策略有效地揭示了高度的替代拼接,增强了我们对植物基因表达的理解.
关键词:
基因表达 基因表达 基因表达基因拼接是基因拼接.这是Iso-Seq.异构体的异构体形式乔乔巴 (Jojoba) 是一种植物.这种植物是Simmondsia chinensis.转录组 转录组就是一个转录组.更多相关视频
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