相关实验视频

Updated: Jul 12, 2025

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes

Published on: April 26, 2019

高自由脂肪酸治疗诱导了自然杀手 (NK) 细胞系中的抗炎变化

Hong Wu^1,2,3, Yanqi Fu^1,2, Yuhuan Jiang⁴

¹Department of Laboratory Medicine, Jiangxi Health Vocational College, Nanchang, Jiangxi 330052, China.

Iranian journal of immunology : IJI

|October 22, 2023

概括

高含量的自由脂肪酸 (FFAs) 显著破坏自然杀手 (NK) 细胞,影响氧化应激和炎症. 在这项研究中,高葡萄糖对NK92细胞没有表现出类似的影响.

科学领域:

免疫学免疫学免疫学
代谢疾病代谢疾病
细胞生物学细胞生物学

背景情况:

自然杀手 (NK) 细胞与与肥胖有关的代谢疾病有关.
在2型糖尿病中NK细胞参与的确切机制尚未完全理解.

研究的目的:

研究高葡萄糖和高自由脂肪酸 (FFAs) 如何影响NK92细胞中的免疫反应,炎症和氧化应激.
阐明这些效应的潜在机制.

主要方法:

NK92细胞被用高葡萄糖或高FFF治疗了4天.
试验包括CCK8细胞毒性,SOD和GSH的生物化学分析,以及ROS,细胞因子 (TNF-α,IFN-γ,IL-6,IL-10) 和细胞内分子 (氨酸,格兰酶B) 的流细胞计.

主要成果:

高水平的FFAs降低了NK92细胞块,ROS生产和关键细胞因子 (TNF-α,IFN-γ,IL-6,IL-10) 的水平.
在高FFA组中,穿孔素和B粒酶的表达也下降了.
高血糖并没有显著改变这些参数.

结论:

高水平的FFF诱导显著的形态变化,并损害NK92细胞中的氧化应激和炎症反应.
这些发现强调了高水平的FFAs对NK细胞功能的有害影响,可能导致代谢功能障碍.

关键词:

细胞因子 (cytokines) 是一种细胞因子.高的FFA高的FFA是什么意思细胞内分子细胞内分子 NK92 细胞 NK92 细胞氧化压力是一种氧化压力.

更多相关视频

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

Published on: February 27, 2014

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase G6PI-Induced RA Mice

Adoptive Immunotherapy of iNKT Cells in Glucose-6-Phosphate Isomerase G6PI-Induced RA Mice

Published on: January 31, 2020

相关概念视频

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...

Inflammation

Inflammation