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对转录组,挥发性和非挥发性代谢物概况的整合揭示了Toona sinensis中特有的芳香形成

Beibei Zhang¹, Lifang Hao², Jing Zhang³

¹College of Forestry, Northwest A&F University, Yangling, Shaanxi, 712100, China; Key Laboratory of Food Safety Risk Assessment, School of Chemistry Engineering, Xian University, Xian, Shaanxi, 710065, China; Shaanxi Key Laboratory of Economic Plant Resources Development and Utilization, Yangling, Shaanxi, 712100, China.

|October 22, 2023

概括

豆的香气是由微绿植物中的挥发性硫化合物 (VSC) 和芽中的烯形成的. 催因子合成酶 (LFS) 影响VSC水平,而IDI和MPDC是基生物合成的关键.

科学领域:

植物生物化学植物生物化学
代谢学代谢学代谢学
文字转录学 (Transcriptomics) 是一个学科.

背景情况:

突尼西斯 (Toona sinensis) 具有独特的香味,但其形成机制尚未完全理解.
芳香化合物显著影响农产品的质量和消费者接受度.

研究的目的:

阐明Tona sinensis芽 (TSB) 和微绿 (TSM) 中芳香形成的分子机制.
确定关键的挥发性化合物和生物合成途径,负责T. sinensis的特征性气味.

主要方法:

用转录组数据对挥发性和非挥发性代谢物的综合分析.
气色谱-质谱 (GC-MS) 用于挥发性物质的分析.
用于基因表达分析的RNA测序.

主要成果:

挥发性硫化合物 (VSC) 在TSM中占主导地位,而烯在TSB中占主导地位.
20种挥发性物质,主要是VSC和烯,被确定为潜在的气味生物标志物.
氨酸中的氨酸合成是TSM的VSC途径的一个关键步骤;S-(trans-l-propenyl) -l-氨酸被确定为可能的VSC前体.
在TSB中高催因子合成酶 (LFS) 表达减少了VSC积累.
异丁二异相酶 (IDI) 和美酸二脱碳酶 (MPDC) 参与了T. sinensis的生物合成.

结论:

这项研究揭示了T. sinensis芽和微绿的独特挥发性配置和生物合成途径,用于T. sinensis芽和微绿的芳香形成.
像LFS,IDI和MPDC这样的关键酶在调节特定芳香化合物的积累方面发挥着至关重要的作用.
这些发现为Toona sinensis aroma.的遗传和代谢基础提供了宝贵的见解.

关键词:

香味的个人资料香味的个人资料不易挥发的代谢物.图纳·辛尼斯斯 (Toona sinensis) 是一个白.转录组转录组就是一个转录组.挥发性代谢物是挥发性的代谢物.

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