对于乳杆菌种类的酸性耐受性. 根病变:一个复杂和多方面的反应
Heitor Sales de Barros Santos1, Nailê Damé-Teixeira2, Martina Hitomi Nagano1
1Preventive and Community Dentistry Department, Dental School, Federal University of Rio Grande do Sul (UFRGS), Ramiro Barcelos, 2492, Porto Alegre 90035-003, Brazil.
Archives of oral biology
|October 22, 2023
概括
乳酸菌种拥有众多的基因,使其能够耐受酸性,这对于它们在与牙相关的酸性口腔环境中生存至关重要. 了解这些适应机制是对抗牙的关键.
科学领域:
- 口腔微生物学 口腔微生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 乳酸菌种是导致牙的关键耐酸细菌.
- 了解它们的酸耐受机制对于制定预防策略至关重要.
研究的目的:
- 鉴定了乳酸菌种中赋予酸耐性的基因.
- 探索声音和根表面的元转录组中的基因功能.
主要方法:
- 生物膜样本的RNA测序 (RNA-seq) 来自声音和的根表面.
- 绘制RNA-seq对口腔微生物基因组进行读取.
- 使用DESeq2.2进行差异基因表达分析.
主要成果:
- 鉴定了15种含有653个过度表达的酸耐受性基因的乳酸菌种在状根表面.
- 与翻译,核糖体生物发生和营养物质运输相关的功能对于酸耐受性至关重要.
- 特定物种的功能有助于Lactobacillus适应在酸性化菌生物膜中的情况.
结论:
- 乳酸菌种对酸性环境表现出复杂,多方面的反应.
- 对适应机制的进一步研究可以为管理牙的新方法提供信息.
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