富罗库马林可以增强皮肤黑色素细胞中UVA诱导的DNA损伤
Megan Hoang1, Abrar Qureshi2, Elena Oancea3
1The Warren Alpert Medical School of Brown University, Providence, RI, USA.
Biochemical and biophysical research communications
|October 22, 2023
概括
饮食中的furocoumarins,如8-methoxypsoralen (8-MOP) 和6等.
科学领域:
- 皮肤病学和致癌发生.
- 光生物学和分子毒理学
背景情况:
- 流行病学研究将高摄入类水果与增加皮肤癌风险联系起来.
- 类水果和一些蔬菜含有furocoumarins,这些化合物通过紫外线相互作用与皮肤癌的发展有关.
研究的目的:
- 为了研究食中的furocoumarins8-methoxypsoralen (8-MOP) 和6',7'-dihydroxybergamottin (DHB) 对UVA诱导的DNA损伤的影响.
- 为了确定这些furocoumarins是否增强人类表皮黑色素细胞的DNA损伤,黑色素瘤起源的细胞.
主要方法:
- 人类表皮黑色素细胞用8-MOP或DHB进行了化.
- 随后,细胞被暴露在物理剂量的UVA辐射中.
- 西方涂抹通过化H2AX (γH2AX) 与总H2AX (γH2AX/H2AX) 的比率来量化DNA损伤.
主要成果:
- 与对照细胞相比,在用DHB治疗的细胞中,UVA诱导的DNA损伤 (γH2AX/H2AX) 的比率显著更高.
- 用8-MOP治疗的细胞显示UVA诱导的DNA损伤显著增加.
- 这些发现表明,furocoumarins可增强黑色细胞中UVA诱导的DNA损伤.
结论:
- 饮食中的furocoumarins,特别是8-MOP和DHB,在人类表皮黑色素细胞中增强UVA诱导的DNA损伤.
- 这种DNA损伤的增强代表了一种潜在的机制,它将类水果的消费和富洛库马林暴露与皮肤癌风险的增加联系起来.
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