相关实验视频

Updated: Jul 12, 2025

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography

Published on: January 18, 2020

一种新型的糖化花生蛋白分离物输送系统的稳定性,该分离物中含有酸

Chenghui Zhang¹, Changzhu Li², Yulin Zhu³

¹School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.

|October 22, 2023

概括

一个新的糖化花生蛋白分离物 (PPI) 输送系统增强了酸 (GA) 应用. 这种新的纳米粒子系统改进了GA.

科学领域:

食品科学与技术食品科学与技术
生物材料工程生物材料工程
营养药物开发营养药物开发

背景情况:

酸 (GA) 具有有益的特性,但面临着应用挑战.
花生蛋白分离物 (PPI) 是一种潜在的生物材料,可用于输送系统.
糖基化可以修改蛋白质的特性,以提高功能.

研究的目的:

为酸 (GA) 开发一种新的基于葡萄糖基化PPI的输送系统.
调查糖化对PPI的结构和功能影响.
为了评估产生的GA载纳米粒子的特性和稳定性.

主要方法:

使用花生蛋白分离物 (PPI) 和酸 (GA) 制备糖化PPI.
纳米粒子大小,多分散性指数 (PDI) 和结构力 (疏水性相互作用) 的表征.
在各种环境条件下评估纳米粒子稳定性.
使用DPPH和ABTS试验评估抗氧化剂活性.

主要成果:

最佳的纳米颗粒是在1:3的糖化PPI与GA比率下形成的.
获得的粒子大小为338.35nm,PDI为0.222.
纳米粒子在不同的环境因素中表现出稳定性.
在108小时后,与自由GA相比,增强了DPPH (35.94%) 和ABTS (62.59%) 的激素清除活动.

结论:

PPI的糖化形成了一种稳定的纳米粒子输送系统,用于酸.
开发的系统显著提高了GA的抗氧化疗效.
这项研究为开发水友多输送系统提供了一个有前途的方法.

关键词:

抗氧化能力抗氧化能力梅拉德反应是梅拉德反应.这是一种PPI-GA结合剂.自组装的纳米粒子.储存的稳定性储存的稳定性

更多相关视频

A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients

A Package of Established Analytical Tools to Investigate the Solid-State Alteration of Lipid-Based Excipients

Published on: August 9, 2022

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

相关概念视频

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Proteoglycans

Proteoglycans

Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...