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Updated: Jul 12, 2025

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

[陶蛋白诱导PS19转基因小鼠的异常替代分离变化]

Xuewen Tian¹, Chan Chen², Xiong Wang¹

¹Department of Clinical Laboratory, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition

|October 22, 2023

概括

在神经退行之前,在tau P301S小鼠中发现了异常的替代拼接 (AS) 事件,特别是跳过的外因子. 分离因子调节失调可能会导致这些AS变化.

科学领域:

神经科学是一个神经科学.
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

病是一种神经退行性疾病,其特点是蛋白聚合.
替代拼接 (AS) 在基因表达调节中起着至关重要的作用.
在AS事件和tau病理进展之间的关系仍然不完全理解.

研究的目的:

调查异常替代拼接 (AS) 事件是否先于PS19小鼠中因tau P301S突变引起的神经退行性表型.
为了识别与病理相关的特定AS事件和受影响的基因.

主要方法:

从PS19小鼠获得的RNA测序数据的大数据分析.
使用rMATS进行AS事件分析和Deseq2/edgeR/limma进行差异性基因表达.
进行了基因本体学 (GO) 丰富,蛋白质-蛋白质相互作用 (PPI) 和基因表达相关性分析.
使用PCR和糖电泳验证的AS事件.

主要成果:

确定了8079个AS事件,其中有117个显著事件 (ΔPSI>0.1).
跳过的exon (SE) 是最常见的AS事件类型 (50.43%).
突触组织和结合体基因在SE和替代3'结合部位 (A3SS) 事件中分别显示出异常结合.
拼接因子Snrpn与许多转录有显著的关联,并且证实了LRp8基因AS.

结论:

失调的拼接因子可能会导致tau P301S诱导的异常AS变化.
这项研究增强了对蛋白和接因子动态在病的理解.

关键词:

另一个替代拼接.在Exon跳过的情况下,Exon跳过.P301SS 没有使用过.突触突触是一个突触突触.病症是一种病症.

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RNA Splicing

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

Alternative RNA Splicing

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...