具有对齐架构和小细胞外囊泡的宏颗粒状水凝具有功能,刺激骨质疏松性肌至骨愈合
Wei Song1, Zhijie Ma2, Xin Wang1
1Department of Orthopedic Surgery, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200233, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|October 22, 2023
概括
一种新的水凝支架 (MHA-sEVs) 在骨质疏松性旋转手套修复中促进肌至骨的愈合. 这种巨孔,对齐的支架增强了组织的整合和生物力学强度,提供了一个有前途的临床策略.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 整形外科手术 整形外科手术
背景情况:
- 在旋转手腕修复 (RCR) 后,骨质疏松性肌至骨愈合 (TBH) 存在重大挑战.
- 现有的支架缺乏对齐的架构,免疫调节能力和有效的TBH所需的巨孔结构.
研究的目的:
- 制造和评估一种新的巨性水凝支架 (MHA-sEVs),其架构与骨质疏松性TBH的免疫调节特性一致.
- 研究脚手架在调节炎症的同时促进细胞透,组织融合和骨再生的能力.
主要方法:
- 从脂肪衍生干细胞中制造一个巨孔水凝支架 (MHA-sEVs),使用酸盐,酸和小细胞外囊泡 (sEVs).
- 在骨质疏松性RCR模型中的体内评估,以评估TBH,组织整合和生物力学强度.
- 关于细胞-凝相互作用的体外研究,包括通过NF-κb通路的基分化和巨分化.
主要成果:
- 由于其巨孔结构,MHA-sEV显著改善了细胞透和组织融合.
- 脚手架通过其对齐的架构和抗炎作用来增强肌修复和骨再生,促进了TBH.
- 与对照组相比,生物力学强度大约增加了两倍,这表明恢复率下降.
- 脚手架对齐诱导了tenogenic分化; sEVs改善了M1巨细胞的线粒体功能,并抑制了M1极化.
结论:
- MHA-sEVs为促进骨质疏松性肌到骨的愈合提供了一个有希望的策略.
- 脚手架对齐的巨孔结构和免疫调节特性是其有效性的关键.
- 这种方法有可能在RCR和其他骨科修复中获得未来的临床应用.
更多相关视频
09:35Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
Published on: September 11, 2015
9.8K
10:18Fragmenting Bulk Hydrogels and Processing into Granular Hydrogels for Biomedical Applications
Published on: May 17, 2022
5.6K
相关概念视频
Bone Remodeling
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
Growth of Cartilage and Bone Tissue
Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...
Osteoclasts in Bone Remodeling
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
The Bone Matrix
Bone contains a relatively small number of cells entrenched in a matrix of collagen fibers that provide an adherent surface for inorganic salt crystals. Both components of the matrix, organic and inorganic, contribute to the unusual properties of bone. Without collagen, bones would be brittle and shatter easily. Without mineral crystals, bones would flex and provide little support. This can be observed by an experiment: when the minerals of a bone are dissolved by soaking the bone in acid or...
Essential Minerals for Bone Health
The minerals contained in all of the food we consume are essential for our organ systems. However, certain essential minerals, such as calcium, phosphorus, magnesium, manganese, and fluoride, largely affect bone health.
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Calcium and Phosphorus
Calcium is a critical component of bones, especially in the form of calcium phosphate and calcium carbonate. Since the body cannot make calcium, it must be obtained from the diet. However, calcium cannot be absorbed from the small intestine without...
Spongy Bone
All bones comprise an outer layer of compact bone, and an interior made up of spongy bone tissue, also called cancellous or trabecular bone. In long bones, spongy bone tissue is mainly found in the interior of the epiphyses (broad ends of the bone).
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...
