相关实验视频

Updated: Jul 12, 2025

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

转录因子Id1通过抑制Tcf3和Tcf4在Th9细胞分化中发挥重要作用

Woo Ho Lee¹, Kyung Jin Hong¹, Hua-Bing Li²

¹Department of Life Science, Sogang University, 35 Baekbeom-ro, Mapo-gu, Seoul, 04107, South Korea.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

|October 22, 2023

概括

Id1是T辅助型9 (Th9) 细胞分化的关键转录因子. 它抑制Tcf3和Tcf4,促进互白素-9的产生,并改善小鼠模型中的喘.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

辅助性T型9 (Th9) 细胞对于免疫反应至关重要,它们产生介白素-9 (IL-9).
尽管涉及多个转录因子,但对Th9细胞分化的转录调节尚未完全理解.

研究的目的:

研究Id1在Th9细胞分化的转录调节中的作用.
阐明Id1控制Th9细胞发育和功能的机制.

主要方法:

通过IL-4和TGF-β诱导Id1.
对Id1缺乏的原始CD4T细胞的分析.
质谱测量用于识别Id1相互作用蛋白.
RNA测序,染色体免疫沉和短暂的记者测试用于研究基因调节.
在喘动物模型中评估Th9细胞功能.

主要成果:

Id1 对于 Th9 细胞分化至关重要; Id1 缺乏会损害这一过程,而 Id1 的过度表达会诱导 IL-9.
在Th9细胞中,Id1与转录因子Tcf3和Tcf4相互作用.
Tcf3和Tcf4通常通过与其促进体结合来抑制IL9基因表达.
Id1 抑制了 Tcf3 和 Tcf4 的活性,从而促进了 Th9 的分化.
在喘模型中,缺少Id1的Th9细胞显示呼吸道炎症减少.

结论:

Id1是一个关键的转录因子,调节Th9细胞分化.
Id1通过抑制Tcf3和Tcf4在Il9基因上的抑制功能来促进Th9细胞分化.
针对Id1可能为喘等炎症性疾病提供治疗策略.

关键词:

Id1 Id1 的意思是这就是Tcf3cf3的意思.这就是Tcf4Tcf4Tcf.在Th9细胞中.不同化的差异化差异化.转录规则转录规则转录规则

更多相关视频

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol

In Vitro Differentiation of Human CD4⁺FOXP3⁺ Induced Regulatory T Cells (iTregs) from Naïve CD4⁺ T Cells Using a TGF-β-containing Protocol

Published on: December 30, 2016

相关概念视频

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

General Transcription Factors

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

Transcription Factors

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...

RNA Polymerase II Accessory Proteins

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...