通过AIPP3-PHD2-CPL2复合体对N-基皮酸生物合成的表观遗传调节
Jiameng Lan1, Siyu Chen1,2, Joana Pico3
1Department of Botany, University of British Columbia, Vancouver, V6T 1Z4, Canada.
Journal of integrative plant biology
|October 23, 2023
概括
植物免疫信号分子N-hydroxypipecolic acid (NHP) 的生物合成是由AIPP3-PHD2-CPL2复合体负面调节的. 这种复杂的表观遗传控制了FLAVIN-DEPENDENT MONOOXYGENASE 1 (FMO1) 基因的表达,影响了植物的防御.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 植物免疫力 植物免疫力
背景情况:
- N-hydroxypipecolic 酸 (NHP) 是植物系统性获得性耐药性 (SAR) 的关键信号分子.
- SAR提供了广泛的,长期的抗病原体防御.
- 了解NHP生物合成调节对于增强植物免疫力至关重要.
研究的目的:
- 为了确定NHP生物合成的负调节剂.
- 研究表观遗传机制在控制NHP水平中的作用.
主要方法:
- 转向基因选用于变异性突变,具有改变的FLAVIN-DEPENDENT MONOOXYGENASE 1 (FMO1) 表达.
- 分析FMO1 (cef) 的构成表达和FMO1 (ief) 突变的诱导表达.
- 研究AIPP3-PHD2-CPL2蛋白质复合物的功能.
主要成果:
- 确定了AIPP3-PHD2-CPL2蛋白质复合体作为FMO1表达的负调节者.
- 证明这个复合体识别了H3K27me3并调解了转录抑制.
- 显示了该复合体在负面调节NHP生物合成中的作用.
结论:
- 由AIPP3-PHD2-CPL2复合体进行表观遗传调节对于控制FMO1表达至关重要.
- 这种表观遗传控制会影响NHP水平,从而影响植物的防御反应.
- 这些发现突出了调节植物免疫力的新机制.
相关概念视频
Biosynthesis of Nucleic Acids
56
Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...
56
Allosteric Proteins-ATCase
5.8K
Binding sites linkages can regulate a protein's function. For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
5.8K
Phosphoinositides and PIPs
8.6K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
8.6K
Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
Covalently Linked Protein Regulators
6.8K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
6.8K
piRNA - Piwi-interacting RNAs
6.9K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.9K


