Circ_0001982通过circ_0001982-miR-144-3p-GSE1轴加剧乳腺癌的发展
Fei Gao1, Jianjun Han1, Li Jia1
1Department of Oncology, The Third Hospital of Mianyang (Sichuan Mental Health Center), Mianyang, Sichuan, China.
Journal of biochemical and molecular toxicology
|October 23, 2023
概括
循环RNAcirc_0001982通过海绵化miR-144-3p和升级GSE1.1,促进乳腺癌 (BC) 的进展. 抑制circ_0001982抑制BC细胞的增殖,糖解,迁移和入侵,为乳腺癌提供潜在的治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 循环RNAs (circRNAs) 正在成为各种癌症的关键调节者,包括乳腺癌 (BC).
- 特定circRNAs的失调可以影响瘤的发展和进展.
- 了解 circRNA 功能背后的分子机制对于识别 BC 中新型治疗点至关重要.
研究的目的:
- 研究circ_0001982在乳腺癌的发展和进展中的作用.
- 阐明circ_0001982影响BC细胞行为的分子机制.
- 探索circ_0001982作为乳腺癌治疗的治疗点的潜力.
主要方法:
- 定量实时聚合酶连锁反应 (qRT-PCR) 和西布洛特用于评估circ_0001982,miR-144-3p和GSE1.1的表达水平.
- 在体外功能测试包括细胞增殖,细胞亡,迁移和入侵测试,以评估细胞行为.
- 使用商业套件进行糖解分析.
- 双露西法酶记者和RNA免疫沉 (RIP) 试验证实circ_0001982,miR-144-3p和GSE1.1之间的相互作用.
- 使用小鼠异种移植模型进行体内研究,以评估circ_0001982对瘤生长的影响.
主要成果:
- Circ_0001982表达与正常对照相比,乳腺癌组织和细胞的表达显著上调.
- 抑制circ_0001982或过度表达miR-144-3p可以抑制BC细胞的增殖,糖解,迁移和入侵,同时促进细胞亡.
- Circ_0001982作为miR-144-3p的分子海绵,GSE1被确定为miR-144-3p的直接目标.
- 通过调节miR-144-3p/GSE1轴,Circ_0001982促进了BC进展,其缺席抑制了体内瘤的生长.
结论:
- Circ_0001982在乳腺癌的发展和进展中起着显著的致癌作用.
- 该circ_0001982/miR-144-3p/GSE1轴是一个关键的调节途径,影响BC细胞增殖,糖解,入侵和亡.
- Circ_0001982代表了乳腺癌的潜在诊断生物标志物和治疗点.
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