用原子力显微镜绘制活细胞内部的压力,以应对环境刺激
Hongxin Wang1, Han Zhang2, Ryo Tamura2
1Research Center for Macromolecules and Biomaterials, National Institute for Materials Science, Tsukuba, Ibaraki, Japan.
Science and technology of advanced materials
|October 23, 2023
概括
这项研究使用原子力显微镜 (AFM) 绘制了活细胞中的细胞内力图. 了解这些力量揭示了细胞如何适应环境变化,有助于癌症等疾病的生存.
科学领域:
- 细胞力学 细胞力学
- 生物物理学的生物物理.
- 细胞骨动力学 细胞骨动力学
背景情况:
- 细胞对刺激的反应决定了细胞的命运,影响了癌症等疾病.
- 细胞内物理力量对于细胞反应至关重要,但需要更好地理解.
- 需要先进的成像来绘制力分布图,并了解细胞机制.
研究的目的:
- 以亚微米分辨率在整个细胞尺度上绘制细胞内力图.
- 为了将细胞内力分布与细胞骨结构相关联.
- 在现场可视化细胞对环境调制的动态机械反应.
主要方法:
- 使用信息学辅助的原子力显微镜 (AFM) 划痕技术.
- 采用马尔科夫链蒙特卡洛优化用于模型和探头几何体的合适.
- 在机械调制下研究了细胞骨和核骨中的力动力学.
主要成果:
- 绘制了细胞内力量的地图,并将它们与细胞骨结构相关联.
- 现场可视化了动态细胞对环境变化的机械反应.
- 在活细胞的细胞骨和核骨中证明了力动力学.
结论:
- 细胞内预压力改变减弱环境刺激.
- 力量动力学与细胞生存有关,而与癌症中机械信号启动的死亡有关.
- 细胞内力量在启动细胞迁移方面发挥着作用.
相关概念视频
01:17Studying the Cytoskeleton
6.3K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
6.3K
01:08Atomic Force Microscopy
3.4K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.4K