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从hiPSC衍生的神经皮质囊产生人类视网膜有机体的可靠性

Madalena Carido¹, Manuela Völkner^1,2, Lisa Maria Steinheuer^3,4

¹Center for Regenerative Therapies Dresden (CRTD), TU Dresden, Dresden, Germany.

Frontiers in cellular neuroscience

|October 23, 2023

概括

该CYST协议有效地从人类诱导多能干细胞 (hiPSCs) 中产生强大的人类视网膜器官 (HROs),提供一种可靠的研究方法. 这种协议产生更多的形,并且比其他方法更稳定.

科学领域:

干细胞生物学干细胞生物学
发育生物学是发展生物学.
眼科医生眼科眼科

背景情况:

来自人类诱导多能干细胞 (hiPSCs) 的人类视网膜器官 (HROs) 对于研究视网膜发育和疾病至关重要.
为了可靠的研究和治疗应用,需要HRO生成的标准化方法.
现有的协议在效率,可复制性和细胞组成方面各不相同.

研究的目的:

评估CYST协议的效率,可靠性和可重复性,用于从hiPSCs生成HRO.
将CYST协议与基于单个hiPSC聚合物的替代方法进行比较.
评估通过不同的协议生成的HRO的稳定性和细胞组成.

主要方法:

使用CYST协议,利用七条不同的hiPSC线路进行HRO生成.
进行了组织学和超结构分析,以评估HRO分化和成熟.
采用单细胞RNA测序和CaSTLe转移学习用于细胞组成分析.
使用四条hiPSC线进行了CYST协议和单一hiPSC聚合协议的比较研究.

主要成果:

CYST协议在不同的hiPSC线路上可重复生成HRO,hiPSC线路是变异的主要来源.
与其他协议相比,CYST衍生的HROs显示出较高比例的体和光受体和Müller glia的平衡比例.
与单一的hiPSC聚合物协议相比,CYST协议显示出更高的HRO产量和长达200天的更高稳定性,该协议表现出病理变化.

结论:

CYST协议是一种高效,可复制和稳定的方法,用于从hiPSCs生成人类视网膜器官.
来自CYST的HRO为基础和转化研究提供了一个有价值的模型系统,具有有利的细胞组成.
这项研究为系统评估和优化有机体协议的未来应用提供了一个框架.

关键词:

滑症是一种滑性疾病.这是 hiPSC.人类人类人类人类人类人类人类神经发育的神经发育这是一种有机物质的有机物质.病理学的病理学视网膜视网膜视网膜是一个干细胞是干细胞的组成部分.

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