MaMADS1-MaNAC083转录调节级联调节香果成熟期间的乙烯生物合成
Wei Wei1, Ying-Ying Yang1, Chao-Jie Wu1
1Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables/Engineering Research Center of Southern Horticultural Products Preservation, Ministry of Education, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Horticulture research
|October 23, 2023
概括
一个新发现的MADS转录因子MaMADS1通过调节乙烯生产来控制香的成熟. 它抑制了MaNAC083,这反过来又控制了乙烯生物合成基因,影响了水果的成熟和储存.
科学领域:
- 植物分子生物学 植物分子生物学
- 果实成熟法规 果实成熟法规
- 控制基因表达 控制基因表达
背景情况:
- 乙烯激素是高潮期水果成熟的关键,比如香.
- 乙烯生物合成的上游转录调节尚未完全理解.
- 在香成熟过程中,乙烯生物合成基因受到上调.
研究的目的:
- 阐明了控制香果成熟过程中的乙烯生物合成的监管级联.
- 为了确定乙烯生物合成基因的上游转录调节器.
- 了解MaNAC083和MaMADS1在这个监管网络中的作用.
主要方法:
- 酵母的一种混合查以确定转录因子结合.
- 在体外和体内实验来确认基因促进剂结合的实验.
- 过时的过度表达实验,以评估对乙烯生产的功能影响.
主要成果:
- MaNAC083直接抑制乙烯生物合成基因 (MaACS1,MaACO1,MaACO4,MaACO5,MaACO8) 的表达.
- MaMADS1直接抑制了MaNAC083的表达,从而缓解了以乙烯生物合成基因的MaNAC083介导的抑制.
- 这就产生了一个MaMADS1-MaNAC083-乙烯生物合成基因调节级联,控制成熟.
结论:
- 一个涉及MaMADS1和MaNAC083的新型调节级联控制了香成熟期间的乙烯生物合成.
- 这项研究加深了对乙烯生物合成的转录调节的理解.
- 这些发现可能有助于制定控制水果成熟和储存的策略.
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