经典单细胞的转录学表征突出显示了免疫炎症在类风湿性关节炎中的骨质侵蚀中的参与
Lucas Peixoto Sales1, Bidossessi Wilfried Hounkpe1, Mariana Ortega Perez1
1Rheumatology Division, Bone Metabolism Laboratory, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil.
Frontiers in immunology
|October 23, 2023
概括
骨质侵蚀的风湿性关节炎患者的古典单细胞显示基因表达发生改变,影响炎症和骨形成. 这些变化凸显了单细胞在RA骨质侵蚀病理生理学中的作用.
科学领域:
- 免疫学 免疫学 免疫学
- 类风湿病学 类风湿病学
- 分子生物学分子生物学
背景情况:
- 经典单细胞在炎症条件下涉及骨质细胞形成和骨质侵蚀.
- 了解风湿性关节炎 (RA) 中的单细胞转录组学对于阐明骨质侵蚀机制至关重要.
研究的目的:
- 在患有侵蚀性与非侵蚀性类风湿性关节炎的患者中分析经典单细胞的转录组概况.
- 识别与RA骨质侵蚀相关的单细胞中差异表达的基因和丰富的通路.
主要方法:
- 进行了RNA测序 (RNA-seq) 在39名绝经前RA患者和健康对照组中分离的古典单细胞上.
- 患者根据手关节骨质侵蚀的存在进行了分层.
- 基因组分析发现了丰富的生物通路.
主要成果:
- 在侵蚀性和非侵蚀性RA组之间确定了1,140个差异表达基因 (DEG).
- 上调的基因包括IL18RAP和KLF14,与免疫反应和炎症有关.
- 下调的基因涉及原代谢 (LARP6) 和骨形成 (PAPPA);与骨质母细胞分化和Wnt信号传递相关的途径在侵蚀性RA中较不活跃.
结论:
- 在RA中单细胞基因表达的改变有助于骨质侵蚀病理生理学.
- 骨形成途径受损和单细胞炎症基因表达增加与RA骨质侵蚀有关.
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