对比转录学揭示了一种新型的表型可塑性形式,通过基因表达的基因特异性变化进化
Andrew J Isdaner1, Nicholas A Levis1,2, David W Pfennig1
1Department of Biology University of North Carolina Chapel Hill North Carolina USA.
Ecology and evolution
|October 23, 2023
概括
新的多功能性是通过血统特定的基因表达进化而来的,而不是通过采用祖先的池干燥反应. 这表明,复杂的可塑性进化需要显著的遗传修饰.
科学领域:
- 进化生物学是进化的生物学.
- 发展性可塑性 发展性可塑性
- 基因组学就是基因组学.
背景情况:
- 现型可塑性可以通过新的遗传变化或通过重新利用现有机制来演变.
- 阿努拉人 (青和) 表现出祖先的可塑性,例如加速幼虫发育,以应对水池干燥等环境线索.
- 资源多元化,就像脚的独特的食用形态一样,代表了复杂形式的表型可塑性.
研究的目的:
- 调查新世界 (属 *Spea*) 中的新型资源多功能性是否通过采用祖先的可塑性机制进化.
- 在不同的池干燥条件下,比较Spea*热带形态和Pelobates*之间的基因表达模式.
主要方法:
- 为*Spea multiplicata*生成了一个de novo转录组,并进行了热带形态之间的差异性基因表达分析.
- 利用现有的基因表达数据和已发表的转录组,对暴露于不同池干燥模式的Pelobates cultripes进行了分析.
- 确定了两个属及其各自的可塑性背景之间的独特和重叠的差异性表达基因.
主要成果:
- 数以千计的差异性表达的基因被确定在Spea multiplicata之间,与显著的发育重组有关.
- 在干燥条件下,大多数在*Spea*中差异表达的基因在*Pelobates*中没有被发现有差异表达.
- 在对池干燥反应的 *Spea* 形态和 *Pelobates* 反应之间观察到基因表达模式的低重叠.
结论:
- 在 *Spea multiplicata* 中的新型资源多功能性似乎主要是通过谱系特定的遗传变化进化而来.
- 与祖先的池干燥可塑性相关的现有基因表达模式的选择在Spea的多性演变中起到了最小的作用.
- 虽然祖先的应激反应可能会启动进化创新,但要发展复杂的表型可塑性,可能需要实质性的谱系特异性修改.
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