描述用于对牛呼吸系统疾病的甲状腺反应的各种抗微生物药物对宿主转录组反应的影响
Rebecca A Bigelow1, John T Richeson1, Molly McClurg2
1Department of Agricultural Sciences, West Texas A&M University, Canyon, TX, United States.
Frontiers in veterinary science
|October 23, 2023
概括
抗微生物药物调节牛的免疫反应. 六种抗微生物药物影响了全血转录组,在料场到达后,在牛群中显示出免疫调节和抗炎作用.
科学领域:
- 兽医免疫学 兽医免疫学
- 文字转录学 (Transcriptomics) 是一个学科.
- 牛呼吸道疾病 (BRD) 研究.
背景情况:
- 牛呼吸道疾病 (BRD) 的控制通常依赖于甲基过敏,即在抵达时大量给予抗菌药物.
- 在甲状腺治疗中使用的不同抗微生物药物的对健康牛的宿主转录组的影响尚未完全理解.
研究的目的:
- 为了研究六种常见的甲基过敏抗菌药物对健康料场牛的全血宿主转录组的影响.
- 为了确定这些抗微生物药物调节的独特和共享的转录基因反应和功能途径.
主要方法:
- 一百五只健康的牛接受了七种治疗之一:负对照或六种不同的抗菌药物.
- 在药物注射后的0,3,7,14,21,56天采集了全血样本.
- 进行了RNA测序和差异基因表达分析,以确定调制的途径.
主要成果:
- 与对照组相比,每个抗微生物药物 (ceftiofur,enrofloxacin,florfenicol,oxytetracycline,tildipirosin,tulathromycin) 都诱导了独特的不同表达的基因.
- 与T细胞受体信号传递,MHC介导的抗原呈现和细胞因子信号传递相关的特定途径在不同时间点被不同的抗微生物药物调节.
- 在第14天,图拉胺显著丰富了与NF-κβ激活和互白素/干扰素信号传递相关的途径.
结论:
- 甲基性抗微生物药物诱导了牛的显著免疫调节,超出了直接的抗微生物效应.
- 这些发现表明,常用的甲状腺药物具有潜在的抗炎和其他治疗作用机制.
- 了解这些转录组变化,可以了解BRD预防策略中的宿主药物相互作用.
相关概念视频
Microorganisms in Medicine and Therapeutics
35
Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
35
Antimicrobial Proteins
1.0K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
1.0K
Antimicrobial Effectiveness
24
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
24
Defense Against Bacterial Pathogens
1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K
Development of Antibiotic Resistance
17
Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
17
Gene Regulation in Microbial Communities: Quorum Sensing
23
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
23


