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根据雌激素周期的状态,大母体 stromal 子宫内膜细胞不同调节炎症:一个体外研究研究

Yat S Wong¹, Ana C Mançanares¹, Felipe I Navarrete¹

¹Laboratory of Animal Biotechnology, Faculty of Veterinary Sciences, Department of Animal Science, Universidad de Concepción, Chillán, Chile.

Frontiers in veterinary science

|October 23, 2023

概括

卵巢周期会影响子宫纤维化. 亲炎性细胞因子在卵泡阶段促进纤维化,而中流阶段通过抗纤维性miRNAs表现出保护作用.

科学领域:

生殖生物学生殖生物学
细胞生物学细胞生物学
纤维化研究纤维化研究

背景情况:

子宫平衡依赖于炎症调节.
卵巢周期对子宫内膜纤维化标志物的影响尚未完全理解.

研究的目的:

在诱导子宫纤维化细胞模型中,研究雌性周期对促炎和抗炎标志物的影响.
分析基因和miRNA的表达,以响应在雌激素周期的不同阶段的亲炎性细胞因子.

主要方法:

来自卵泡和中流体阶段的母体内膜膜层细胞被TGFβ和促炎细胞因子 (IL1β,IL6,TNFα) 治疗.
用实时定量PCR (RT-qPCR) 分析了基因和miRNA表达.
还评估了细胞外囊泡中的miRNA含量.

主要成果:

毛囊相细胞暴露于细胞因子时显示出原和介质基因的表达增加,以及亲纤维的miRNAs (miR17,miR21,miR433).
中阶段细胞表现出细胞外矩阵处理基因 (MMP2,MMP9) 和抗纤维小RNA (miR26a,miR29b,miR29c,miR145,miR378,mir488) 的过度表达.
细胞外囊泡反映了细胞miRNA趋势,在卵泡阶段下调的抗纤维素和上调的亲纤维素miRNA.

结论:

促炎性细胞因子在卵泡阶段放大TGFβ信号,导致纤维化标志物增加和细胞外矩阵组件的不平衡.
中间形阶段显示出对纤维化有保护作用,由抗纤维化miRNAs的增加和纤维化相关基因表达的减少介导.

关键词:

在TGFβ信号通路中.这是一种抗纤维性miRNA.子宫内膜的 stromal 细胞.在子宫内膜异位 (endometrosis) 中.细胞外囊泡中的细胞外囊泡.与纤维化相关的基因一个亲纤维的miRNA.

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